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Literature summary extracted from

  • DeLeo, A.B.; Dayan, J.; Sprinson, D.B.
    Purification and kinetics of tyrosine-sensitive 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthetase from Salmonella (1973), J. Biol. Chem., 248, 2344-2353.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 Co2+ concentrations above 0.1 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.54 EDTA no inhibition Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.54 phosphate phosphate buffer destabilizes Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 additional information
-
 additional information kinetics Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.54 0.013
-
 D-erythrose 4-phosphate pH 7.4, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.54 0.015
-
 phosphoenolpyruvate pH 7.4, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Co2+ not required, Co2+ is inhibitory at concentrations above 0.1 mM Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 100000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Salmonella enterica subsp. enterica serovar Typhimurium first enzyme of the aromatic amino acid biosynthesis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Salmonella enterica subsp. enterica serovar Typhimurium
-
 tyrosine-sensitive isozyme
-
2.5.1.54 Salmonella enterica subsp. enterica serovar Typhimurium
-
 operator-constitutive strain tyrO
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate sequential mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate ordered ping-pong bi-bi mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 18.5
-
 purified enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 r
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O first enzyme of the aromatic amino acid biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7.4
-
 Hepes buffer Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.54 additional information
-
 additional information product inhibition pattern Salmonella enterica subsp. enterica serovar Typhimurium