EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.54 | dithiothreitol | stimulates | Kitasatospora aureofaciens |
EC Number | General Stability | Organism |
---|---|---|
2.5.1.54 | dithiothreitol stabilizes during incubation at 30°C for 15 min | Kitasatospora aureofaciens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.54 | EDTA | reversible by Co2+ | Kitasatospora aureofaciens | |
2.5.1.54 | p-hydroxymercuribenzoate | - |
Kitasatospora aureofaciens | |
2.5.1.54 | Phe | no inhibition | Kitasatospora aureofaciens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.54 | 0.16 | - |
D-erythrose 4-phosphate | 30°C, pH 7.0 | Kitasatospora aureofaciens | |
2.5.1.54 | 0.3 | - |
phosphoenolpyruvate | 30°C, pH 7.0 | Kitasatospora aureofaciens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.54 | Co2+ | stimulates | Kitasatospora aureofaciens | |
2.5.1.54 | Co2+ | required for full activity | Kitasatospora aureofaciens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 97000 | - |
gel filtration | Kitasatospora aureofaciens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Kitasatospora aureofaciens | first enzyme of the aromatic amino acid biosynthesis | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Kitasatospora aureofaciens Tu24 | first enzyme of the aromatic amino acid biosynthesis | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.54 | Kitasatospora aureofaciens | - |
strain Tü 24 | - |
2.5.1.54 | Kitasatospora aureofaciens Tu24 | - |
strain Tü 24 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.54 | 170fold | Kitasatospora aureofaciens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | ordered ping-pong bi-bi mechanism | Kitasatospora aureofaciens | |
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | first substrate is phosphoenolpyruvate | Kitasatospora aureofaciens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 1.3 | - |
partially purified enzyme | Kitasatospora aureofaciens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Kitasatospora aureofaciens | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | first enzyme of the aromatic amino acid biosynthesis | Kitasatospora aureofaciens | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Kitasatospora aureofaciens Tu24 | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
2.5.1.54 | phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | first enzyme of the aromatic amino acid biosynthesis | Kitasatospora aureofaciens Tu24 | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 30 | - |
assay at | Kitasatospora aureofaciens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | additional information | - |
phosphoenolpyruvate protects against inactivation | Kitasatospora aureofaciens |
2.5.1.54 | additional information | - |
D-erythrose 4-phosphate, erythrose and ribose 5-phosphate increase rate of inactivation | Kitasatospora aureofaciens |
2.5.1.54 | 30 | - |
pH 6.0-8.0, stable for at least 2 h | Kitasatospora aureofaciens |
2.5.1.54 | 50 | - |
inactivation above | Kitasatospora aureofaciens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 7 | - |
- |
Kitasatospora aureofaciens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 6.2 | 7.9 | pH 6.2: about 35% of maximal activity, pH 7.9: about 55% of maximal activity | Kitasatospora aureofaciens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.54 | 6 | 8 | 30°C, stable for at least 2 h | Kitasatospora aureofaciens |
2.5.1.54 | 7 | 7.5 | maximal stability | Kitasatospora aureofaciens |