EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.43 | 30000 | - |
x * 30000, SDS-PAGE | Hordeum vulgare |
2.5.1.43 | 40000 | 50000 | gel filtration | Hordeum vulgare |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.43 | S-adenosyl-L-methionine | Hordeum vulgare | synthesis of nicotianamide, the key precursor of the mugineic acid family phytosiderophores | ? | - |
? | |
2.5.1.43 | S-adenosyl-L-methionine | Hordeum vulgare | strongly induced by Fe-deficiency treatment | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.43 | Hordeum vulgare | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.43 | - |
Hordeum vulgare |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.43 | root | primary roots | Hordeum vulgare | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.43 | additional information | - |
- |
Hordeum vulgare |
EC Number | Storage Stability | Organism |
---|---|---|
2.5.1.43 | 4°C, in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, stable for about 1 week | Hordeum vulgare |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.43 | S-adenosyl-L-methionine | - |
Hordeum vulgare | 5'-S-methyl-5'-thioadenosine + nicotianamine | - |
? | |
2.5.1.43 | S-adenosyl-L-methionine | synthesis of nicotianamide, the key precursor of the mugineic acid family phytosiderophores | Hordeum vulgare | ? | - |
? | |
2.5.1.43 | S-adenosyl-L-methionine | strongly induced by Fe-deficiency treatment | Hordeum vulgare | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.43 | ? | x * 30000, SDS-PAGE | Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.43 | 9 | - |
- |
Hordeum vulgare |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.43 | 4 | - |
overnight in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, 90% loss of activity | Hordeum vulgare |
2.5.1.43 | 5.5 | - |
overnight in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, 80% loss of activity | Hordeum vulgare |
2.5.1.43 | 7 | - |
activity is almost completely recovered after overnight exposure in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl | Hordeum vulgare |