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Literature summary extracted from

  • Gefter, M.L.
    T3 and UV-T3-induced S-adenosylmethionine cleavage enzyme (Escherichia coli) (1971), Methods Enzymol., 17 B, 406-411.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.5.1.4 not stable to repeated freezing and thawing Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.4 Mn2+
-
 Escherichia coli
2.5.1.4 additional information no effect: Mg2+, EDTA Escherichia coli
2.5.1.4 S-adenosyl-L-ethionine racemic mixture, 0.2 mM, 50% inhibition Escherichia coli
2.5.1.4 S-adenosyl-L-homocysteine
-
 Escherichia coli
2.5.1.4 S-Ribosylmethionine
-
 Escherichia coli
2.5.1.4 SH-binding reagents
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.4 1
-
 S-adenosyl-L-methionine
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.4 Escherichia coli
-
 infected with bacteriophage T3 and UV-T3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.4 method that includes chromatography on DEAE-cellulose Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.4 3.07
-
 E. coli infected with bacteriophage T3 Escherichia coli
2.5.1.4 28.3
-
 E. coli infected with bacteriophage UV-T3 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.5.1.4 -10°C, enzyme from E. coli infected with bacteriophage UV-T3 is stable for 2 weeks Escherichia coli
2.5.1.4 0°C or -10°C, enzyme from E. coli infected with bacteriophage T3 loses all of its activity within 3 days Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.4 S-adenosyl-L-methionine
-
 Escherichia coli 5'-methylthioadenosine + 2-aminobutan-4-olide
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.4 8.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.4 6 9 about 60% of activity maximum at pH 6 and 9 Escherichia coli