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Literature summary extracted from

  • Kim, Y.S.; Nosaka, K.; Downs, D.M.; Kwak, J.M.; Park, D.; Chung, I.K.; Nam, H.G.
    A Brassica cDNA clone encoding a bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase involved in thiamin biosynthesis (1998), Plant Mol. Biol., 37, 955-966.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.3 bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity, expression in Escherichia coli Brassica napus
2.7.1.49 cDNA clone pBTH1 encoding bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase established in Escherichia coli DH5alpha, expressed in Escherichia coli NI500 and Salmonella typhimurium DM456 by functional complementation Brassica napus
2.7.1.49 single-copy gene in BAC clone T23L3 Arabidopsis thaliana
2.7.4.7 cloning of BTH1 cDNA in Escherichia coli Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.49 plastid
-
 Arabidopsis thaliana 9536
-
2.7.1.49 plastid
-
 Brassica napus 9536
-
2.7.4.7 plastid putative plastid target signal in BTH1 suggests plastidic localization Brassica napus 9536
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.3 2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate Brassica napus involved in biosynthesis of thiamine diphosphate + ?
-
 ?
2.5.1.3 2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate Brassica napus expression is negatively regulated by thiamin diphosphate + ?
-
 ?
2.7.1.49 ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine Arabidopsis thaliana involved in thiamine biosynthetic pathway, first steps ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
2.7.1.49 ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine Brassica napus involved in thiamine biosynthetic pathway, first steps ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
2.7.4.7 ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine Brassica napus enzyme has both 2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate kinase and thiamin-phosphate diphosphatase activities, involved in thiamine biosynthesis ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.3 Brassica napus
-
 bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity
-
2.7.1.49 Arabidopsis thaliana
-
-
-
2.7.1.49 Brassica napus O48881 rape
-
2.7.4.7 Brassica napus O48881
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.49 leaf
-
 Brassica napus
-
2.7.1.49 root
-
 Brassica napus
-
2.7.1.49 stem
-
 Brassica napus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.3 2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate involved in biosynthesis of thiamine Brassica napus diphosphate + ?
-
 ?
2.5.1.3 2-methyl-4-amino-5-diphosphomethylpyrimidine + 2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate expression is negatively regulated by thiamin Brassica napus diphosphate + ?
-
 ?
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
-
 Brassica napus thiamine monophosphate + diphosphate
-
 ?
2.7.1.49 ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
 Arabidopsis thaliana ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
2.7.1.49 ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
 Brassica napus ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
2.7.1.49 ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine involved in thiamine biosynthetic pathway, first steps Arabidopsis thaliana ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
2.7.1.49 ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine involved in thiamine biosynthetic pathway, first steps Brassica napus ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
2.7.1.49 additional information bifunctional hydroxypyrimidine kinase/thiamin-phosphate pyrophosphorylase Brassica napus ?
-
 ?
2.7.4.7 ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine enzyme has both 2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate kinase and thiamin-phosphate diphosphatase activities Brassica napus ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine
-
 ?
2.7.4.7 ATP + 2-methyl-4-amino-5-phosphomethylpyrimidine enzyme has both 2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate kinase and thiamin-phosphate diphosphatase activities, involved in thiamine biosynthesis Brassica napus ADP + 2-methyl-4-amino-5-diphosphomethylpyrimidine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.7 More enzyme has 2 distinct domains related to TMP-PPase and HMP-P kinase activities Brassica napus