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Literature summary extracted from

  • Reddick, J.J.; Nicewonger, R.; Begley, T.P.
    Mechanistic studies on thiamin phosphate synthase: evidence for a dissociative mechanism (2001), Biochemistry, 40, 10095-10102.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.3 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.3 from E. coli overexpression strain Bacillus subtilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.3 4-amino-2-methyl-5-(diphosphomethyl)pyrimidine + 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate = diphosphate + thiamine phosphate + CO2 dissociative SN1 mechanism Bacillus subtilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.3 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole
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 Bacillus subtilis thiamine monophosphate + diphosphate
-
 ?
2.5.1.3 4-amino-5-(hydroxymethyl)-2-(trifluoromethyl)pyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole poor substrate Bacillus subtilis ?
-
 ?
2.5.1.3 4-amino-5-(hydroxymethyl)-2-methoxypyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)-thiazole good substrate Bacillus subtilis ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.3 additional information
-
 additional information
-
 Bacillus subtilis