Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Graser, G.; Hartmann T.
    Biosynthesis of spermidine, a direct precursor of pyrrolizidine alkaloids in root cultures of Senecio vulgaris L. (2000), Planta, 211, 239-245.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 1,3-diaminopropane 96% inhibition at 0.01 mM and 100% inhibition at 0.1 mM Senecio vulgaris
2.5.1.16 5'-methylthioadenosine 83% inhibition at 0.01 mM and 22% inhibition at 1 mM Senecio vulgaris
2.5.1.16 agmatine 89% inhibition at 0.1 mM and 61% inhibition at 1 mM Senecio vulgaris
2.5.1.16 arginine 98% inhibition at 0.01 mM, 95% inhibition at 0.1 mM and 84% inhibition at 1 mM Senecio vulgaris
2.5.1.16 cadaverine 77% inhibition at 0.01 mM and 22% inhibition at 1 mM Senecio vulgaris
2.5.1.16 Cyclohexylamine
-
 Senecio vulgaris
2.5.1.16 Homospermidine 95% inhibition at 0.01 mM, 92% inhibition at 0.1 mM and 30% inhibition at 1 mM Senecio vulgaris
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine complete inhibition at 0.080 mM Senecio vulgaris
2.5.1.16 spermidine product inhibition at physiological concentrations Senecio vulgaris
2.5.1.16 spermine 99% inhibition at 0.1 mM and 90% inhibition at 1 mM Senecio vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 0.004
-
 S-adenosyl-(5')-3-methylthio-1-propylamine pH 7.5, 34ºC Senecio vulgaris
2.5.1.16 0.021
-
 1,4-diaminobutane pH 7.5, 34ºC Senecio vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 37000
-
 gel filtration Senecio vulgaris

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Senecio vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 partial, streptomycin sulfate precipitation, ammonium sulfate fractionation and gel filtration, 18fold purified Senecio vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.16 root 10 days old Senecio vulgaris
-

Storage Stability

EC Number Storage Stability Organism
2.5.1.16 -20ºC, partially purified enzyme, 0.1 M NaCl and 1% bovine serum albumin, loss of 50% of activity after 15 days Senecio vulgaris
2.5.1.16 4ºC, crude extracts, loss of activity within 24 hours Senecio vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Senecio vulgaris 5'-methylthioadenosine + spermidine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.16 34
-
 assay at Senecio vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.16 37
-
 above, progressive inactivation Senecio vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.16 7.7
-
 in Gly-NaOH buffer Senecio vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.16 additional information
-
 half-maximal activities at pH 7.2 and 8.4 Senecio vulgaris