BRENDA - Enzyme Database

Biosynthesis of spermidine, a direct precursor of pyrrolizidine alkaloids in root cultures of Senecio vulgaris L.

Graser, G.; Hartmann T.; Planta 211, 239-245 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.16
1,3-Diaminopropane
96% inhibition at 0.01 mM and 100% inhibition at 0.1 mM
Senecio vulgaris
2.5.1.16
5'-methylthioadenosine
83% inhibition at 0.01 mM and 22% inhibition at 1 mM
Senecio vulgaris
2.5.1.16
agmatine
89% inhibition at 0.1 mM and 61% inhibition at 1 mM
Senecio vulgaris
2.5.1.16
arginine
98% inhibition at 0.01 mM, 95% inhibition at 0.1 mM and 84% inhibition at 1 mM
Senecio vulgaris
2.5.1.16
cadaverine
77% inhibition at 0.01 mM and 22% inhibition at 1 mM
Senecio vulgaris
2.5.1.16
Cyclohexylamine
-
Senecio vulgaris
2.5.1.16
Homospermidine
95% inhibition at 0.01 mM, 92% inhibition at 0.1 mM and 30% inhibition at 1 mM
Senecio vulgaris
2.5.1.16
S-adenosyl-(5')-3-methylthio-1-propylamine
complete inhibition at 0.080 mM
Senecio vulgaris
2.5.1.16
spermidine
product inhibition at physiological concentrations
Senecio vulgaris
2.5.1.16
spermine
99% inhibition at 0.1 mM and 90% inhibition at 1 mM
Senecio vulgaris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.16
0.004
-
S-adenosyl-(5')-3-methylthio-1-propylamine
pH 7.5, 34ºC
Senecio vulgaris
2.5.1.16
0.021
-
1,4-diaminobutane
pH 7.5, 34ºC
Senecio vulgaris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.16
37000
-
gel filtration
Senecio vulgaris
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.16
Senecio vulgaris
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.5.1.16
partial, streptomycin sulfate precipitation, ammonium sulfate fractionation and gel filtration, 18fold purified
Senecio vulgaris
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.16
root
10 days old
Senecio vulgaris
-
Storage Stability
EC Number
Storage Stability
Organism
2.5.1.16
-20ºC, partially purified enzyme, 0.1 M NaCl and 1% bovine serum albumin, loss of 50% of activity after 15 days
Senecio vulgaris
2.5.1.16
4ºC, crude extracts, loss of activity within 24 hours
Senecio vulgaris
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.16
34
-
assay at
Senecio vulgaris
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.5.1.16
37
-
above, progressive inactivation
Senecio vulgaris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.16
7.7
-
in Gly-NaOH buffer
Senecio vulgaris
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.5.1.16
additional information
-
half-maximal activities at pH 7.2 and 8.4
Senecio vulgaris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.5.1.16
1,3-Diaminopropane
96% inhibition at 0.01 mM and 100% inhibition at 0.1 mM
Senecio vulgaris
2.5.1.16
5'-methylthioadenosine
83% inhibition at 0.01 mM and 22% inhibition at 1 mM
Senecio vulgaris
2.5.1.16
agmatine
89% inhibition at 0.1 mM and 61% inhibition at 1 mM
Senecio vulgaris
2.5.1.16
arginine
98% inhibition at 0.01 mM, 95% inhibition at 0.1 mM and 84% inhibition at 1 mM
Senecio vulgaris
2.5.1.16
cadaverine
77% inhibition at 0.01 mM and 22% inhibition at 1 mM
Senecio vulgaris
2.5.1.16
Cyclohexylamine
-
Senecio vulgaris
2.5.1.16
Homospermidine
95% inhibition at 0.01 mM, 92% inhibition at 0.1 mM and 30% inhibition at 1 mM
Senecio vulgaris
2.5.1.16
S-adenosyl-(5')-3-methylthio-1-propylamine
complete inhibition at 0.080 mM
Senecio vulgaris
2.5.1.16
spermidine
product inhibition at physiological concentrations
Senecio vulgaris
2.5.1.16
spermine
99% inhibition at 0.1 mM and 90% inhibition at 1 mM
Senecio vulgaris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.16
0.004
-
S-adenosyl-(5')-3-methylthio-1-propylamine
pH 7.5, 34ºC
Senecio vulgaris
2.5.1.16
0.021
-
1,4-diaminobutane
pH 7.5, 34ºC
Senecio vulgaris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.5.1.16
37000
-
gel filtration
Senecio vulgaris
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.16
partial, streptomycin sulfate precipitation, ammonium sulfate fractionation and gel filtration, 18fold purified
Senecio vulgaris
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.16
root
10 days old
Senecio vulgaris
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.5.1.16
-20ºC, partially purified enzyme, 0.1 M NaCl and 1% bovine serum albumin, loss of 50% of activity after 15 days
Senecio vulgaris
2.5.1.16
4ºC, crude extracts, loss of activity within 24 hours
Senecio vulgaris
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.16
34
-
assay at
Senecio vulgaris
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.5.1.16
37
-
above, progressive inactivation
Senecio vulgaris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.16
7.7
-
in Gly-NaOH buffer
Senecio vulgaris
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.5.1.16
additional information
-
half-maximal activities at pH 7.2 and 8.4
Senecio vulgaris