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Literature summary extracted from

  • Sindhu, R.K.; Cohen, S.S.
    Putrescine aminopropyltransferase (spermidine synthase) of Chinese Cabbage (1983), Methods Enzymol., 94, 279-285.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.5.1.16 dialysis against glycine-NaOH buffer, pH 8.8 inactivates Brassica rapa subsp. pekinensis
2.5.1.16 DTT and/or EDTA do not stabilize Brassica rapa subsp. pekinensis
2.5.1.16 heat-inactivated enzyme preparation does not restore the activity Brassica rapa subsp. pekinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 81000
-
 gel filtration Brassica rapa subsp. pekinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Escherichia coli
-
 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Brassica rapa subsp. pekinensis
-
 5'-methylthioadenosine + spermidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Brassica rapa subsp. pekinensis
-
 Chinese cabbage, var Pak Choy
-
2.5.1.16 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 streptomycin and ammonium sulfate fractionation, acetone precipitation and chromatography on Sephadex G-100, 160fold purified Brassica rapa subsp. pekinensis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.16 S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine first discovered Escherichia coli
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.16 leaf
-
 Brassica rapa subsp. pekinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.16 1.28
-
-
 Brassica rapa subsp. pekinensis

Storage Stability

EC Number Storage Stability Organism
2.5.1.16 -15°C, disrupted protoplasts lose more than 90% of activity within 1 week Brassica rapa subsp. pekinensis
2.5.1.16 -20°C, about 30% loss of activity in the final enzyme preparation within 1 month Brassica rapa subsp. pekinensis
2.5.1.16 -20ºC, stable after the acetone precipitation Brassica rapa subsp. pekinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Escherichia coli 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Brassica rapa subsp. pekinensis 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Escherichia coli 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.16 S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Brassica rapa subsp. pekinensis 5'-methylthioadenosine + spermidine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.16 37
-
 assay at Escherichia coli
2.5.1.16 37
-
 assay at Brassica rapa subsp. pekinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.16 additional information
-
 at a given pH, the activity of the enzyme in Tris buffer is lower than in phosphate or Gly-NaOH buffers Brassica rapa subsp. pekinensis
2.5.1.16 8.8
-
 in Gly-NaOH buffer Brassica rapa subsp. pekinensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.16 6
-
 no activity detected in phosphate buffer Brassica rapa subsp. pekinensis
2.5.1.16 10
-
 no activity detected in Gly-NaOH buffer Brassica rapa subsp. pekinensis