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Literature summary extracted from

  • Rebeille, F.; Macherel, D.; Mouillon, J.M.; Garin, J.; Douce, R.
    Folate biosynthesis in higher plants: purification and molecular cloning of a bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase localized in mitochondria (1997), EMBO J., 16, 947-957.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.15 7,8-Dihydropteroic acid
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.15 0.0006
-
 p-Aminobenzoic acid
-
 Pisum sativum
2.5.1.15 0.03
-
 6-hydroxymethyldihydropteridine diphosphate
-
 Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.15 mitochondrion
-
 Pisum sativum 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.15 Mg2+ required to synthesize product Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.15 280000 300000 gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Pisum sativum
-
 diphosphate + 7,8-dihydropteroate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.15 anion exchange and affinity column Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.15 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.15 0.425
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
 Pisum sativum diphosphate + 7,8-dihydropteroate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.15 30
-
 assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.15 9
-
-
 Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.15 0.005 0.008 7,8-dihydropteroate inhibitor of p-aminobenzoic acid Pisum sativum
2.5.1.15 0.007 0.011 7,8-dihydropteroate inhibitor of 6-hydroxymethyl-7,8-dihydropteridine diphosphate Pisum sativum