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Literature summary extracted from

  • Eppler, C.M.; Morré, D.J.; Keenan, T.W.
    Ganglioside biosynthesis in rat liver: characterization of cytidine-5-monophospho-n-acetylneuraminic acid:hematoside (GM3) sialyltransferase (1980), Biochim. Biophys. Acta, 619, 318-331.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.8 Digitonin
-
 Rattus norvegicus
2.4.3.8 Triton CF-54 activation, further enhanced by supplementation with diacyl phospholipids Rattus norvegicus
2.4.3.8 Triton X-100 activation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.8 EDTA Mg2+ protects Rattus norvegicus
2.4.3.8 Lysophospholipids
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.8 0.2
-
 ganglioside GM3
-
 Rattus norvegicus
2.4.3.8 0.8
-
 CMP-N-acetylneuraminate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.8 Golgi apparatus
-
 Rattus norvegicus 5794
-
2.4.3.8 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.3.8 Mg2+ stimulation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.8 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.3.8 glycoprotein
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.8 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.8 0.00105
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.8 CMP-alpha-N-acetylneuraminate + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 ?
2.4.3.8 CMP-N-acetylneuraminate + N-alpha-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. disialosyllactosylceramide or ganglioside GD3 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.3.8 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.8 6.2
-
-
 Rattus norvegicus