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Literature summary extracted from

  • Hori, N.; Watanabe, M.; Yamazaki, Y.; Mikami, Y.
    Purification and characterization of thermostable pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus (1990), Agric. Biol. Chem., 54, 763-768.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.2 0.19
-
 uridine
-
 Geobacillus stearothermophilus
2.4.2.2 0.32
-
 5-methyluridine
-
 Geobacillus stearothermophilus
2.4.2.2 0.46
-
 thymidine
-
 Geobacillus stearothermophilus
2.4.2.2 0.58
-
 2'-deoxyuridine
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.2 54000
-
 2 * 54000, SDS-PAGE Geobacillus stearothermophilus
2.4.2.2 85000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.2 Geobacillus stearothermophilus
-
 JTS 859
-
2.4.2.2 Geobacillus stearothermophilus JTS 859
-
 JTS 859
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.2
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.2 99.6
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.2 5-methyluridine + phosphate
-
 Geobacillus stearothermophilus 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 5-methyluridine + phosphate
-
 Geobacillus stearothermophilus JTS 859 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 deoxyuridine + phosphate 2'-deoxyuridine Geobacillus stearothermophilus uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 deoxyuridine + phosphate 2'-deoxyuridine Geobacillus stearothermophilus JTS 859 uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 thymidine + phosphate
-
 Geobacillus stearothermophilus thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 thymidine + phosphate
-
 Geobacillus stearothermophilus JTS 859 thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Geobacillus stearothermophilus uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Geobacillus stearothermophilus JTS 859 uracil + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.2 dimer 2 * 54000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.2 70
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.2 70
-
 half-life: 25 min in 20 mM potassium phosphate, 15.1 h in 20 mM potassium phosphate and 1 mM 5-methyluridine Geobacillus stearothermophilus
2.4.2.2 80
-
 1 h, 20 mM potassium phosphate, complete inactivation Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.2 7 11.5
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.2 6 12 pH 6.0: about 75% of maximal activity, pH 12.0: about 80% of maximal activity Geobacillus stearothermophilus