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Literature summary extracted from

  • Li, L.; Karlsson, O.P.; Wieslander, A.
    Activating amphiphiles cause a conformational change of the 1,2-diacylglycerol 3-glucosyltransferase from Acholeplasma laidlawii membranes according to proteolytic digestion (1997), J. Biol. Chem., 272, 29602-29606.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.337 dodecylphosphoglycerol anionic amphiphiles are essential for the restoration of a proper conformation. Amphiphilic environment with a critical fraction of negatively charged headgroups induces a catalytic, active site conformation of the enzyme Acholeplasma laidlawii
2.4.1.337 dodecylphosphoglycerol can stimulate the MGlcDAG synthase activity efficiently with a concomitant protection toward proteolytic digestion Acholeplasma laidlawii
2.4.1.337 phosphatidylglycerol anionic amphiphiles are essential for the restoration of a proper conformation. Amphiphilic environment with a critical fraction of negatively charged headgroups induces a catalytic, active site conformation of the enzyme Acholeplasma laidlawii

General Stability

EC Number General Stability Organism
2.4.1.337 total digestion by proteinase K, trypsin or chymotrypsin when no lipid is present, the activity is highly restored in micelles containing 1,2-dioleoyl-phosphatidylglycerol and 1,2-dioleoyl-phosphatidylserine Acholeplasma laidlawii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.337 membrane
-
 Acholeplasma laidlawii 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.337 UDPglucose + 1,2-diacylglycerol Acholeplasma laidlawii the enzyme synthesizes the major nonbilayer-prone lipid monoglucosyldiacylglycerol in the membrane, which is important for spontaneous curvature, and is an important site for the lipid surface charge density UDP + 3-D-glucosyl-1,2-diacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.337 Acholeplasma laidlawii
-
-
-
2.4.1.337 Acholeplasma laidlawii Q93P60
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.337 proteolytic resistance shows a good correlation with the enzyme activity in various lipid-CHAPS mixed micelles. Anionic lipids 1,2-dioleoyl-phosphatidylglycerol and 1,2-dioleoyl-posphatidylserine are able to protect the exposed MGlc-DAG synthase from digestion, whereas 1,2-dioleoyl-phosphatidylcholine and diglucosyldiacylglycerol can not. The detergent dodecylphosphoglycerol can also stimulate the MGlcDAG synthase activity efficiently with a concomitant protection toward proteolytic digestion Acholeplasma laidlawii

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.4.1.337 critical fractions of anionic phospholipids 1,2-dioleoyl-phosphatidylglycerol and 1,2-dioleoyl-posphatidylserine are essential for the restoration of enzyme activity, while the zwitterionic 1,2-dioleoyl-phosphatidylcholine and the uncharged diglucosyldiacylglycerol are not Acholeplasma laidlawii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.337 UDPglucose + 1,2-diacylglycerol
-
 Acholeplasma laidlawii UDP + 3-D-glucosyl-1,2-diacylglycerol
-
 ?
2.4.1.337 UDPglucose + 1,2-diacylglycerol the enzyme synthesizes the major nonbilayer-prone lipid monoglucosyldiacylglycerol in the membrane, which is important for spontaneous curvature, and is an important site for the lipid surface charge density Acholeplasma laidlawii UDP + 3-D-glucosyl-1,2-diacylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.337 MGlcDAG synthase
-
 Acholeplasma laidlawii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.337 28
-
 half-life in 1,2-dioleoylphosphatidylglycerol-CHAPS micelles is more than 2 h, half-life is less than 0.5 h in 1,2-dioleoylphosphatidylcholine-CHAPS micelles Acholeplasma laidlawii