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Literature summary extracted from

  • Entsch, B.; Parker, C.W.; Letham, D.S.; Summons, R.E.
    Preparation and characterization, using high-performance liquid chromatography, of an enzyme forming glucosides of cytokinins (1979), Biochim. Biophys. Acta, 570, 124-139.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.118 0.15
-
 zeatin
-
 Raphanus sativus
2.4.1.118 0.19
-
 UDPglucose
-
 Raphanus sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.118 46500
-
 gel filtration Raphanus sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.118 UDPglucose + N6-alkylaminopurine Raphanus sativus involved in regulation of cytokinin activity UDP + N6-alkylaminopurine-7-beta-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.118 Raphanus sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.118 partial Raphanus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.118 cotyledon
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.118 0.00046
-
-
 Raphanus sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.118 TDP-glucose + zeatin as effective as UDPglucose Raphanus sativus TDP + zeatin 7-beta-D-glucoside
-
 ?
2.4.1.118 UDP-alpha-D-glucose + trans-zeatin i.e. N6-(4-hydroxy-3-methylbut-trans-2-enylamino)purine Raphanus sativus UDP + trans-zeatin 7-beta-D-glucoside + zeatin 9-glucoside ?
2.4.1.118 UDPglucose + 6-benzylaminopurine activity is 15% higher for 6-benzylaminopurine than for zeatin Raphanus sativus UDP + 6-benzylaminopurine-7-beta-D-glucoside
-
 ?
2.4.1.118 UDPglucose + dihydroxyzeatin
-
 Raphanus sativus UDP + dihydroxyzeatin 7-beta-D-glucoside
-
 ?
2.4.1.118 UDPglucose + N6-alkylaminopurine
-
 Raphanus sativus UDP + N6-alkylaminopurine-7-beta-D-glucoside
-
 ?
2.4.1.118 UDPglucose + N6-alkylaminopurine involved in regulation of cytokinin activity Raphanus sativus UDP + N6-alkylaminopurine-7-beta-D-glucoside
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.118 7.3 7.4
-
 Raphanus sativus