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Literature summary extracted from

  • Connett, R.J.; Kirshner, N.
    Purification and properties of bovine phenylethanolamine N-methyltransferase (1970), J. Biol. Chem., 245, 329-334.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.28 p-hydroxymercuribenzoate 0.00001 mM, 75% loss of activity Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.28 38000
-
 sucrose density gradient centrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.28 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.28
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.28 S-adenosyl-L-methionine + phenylethanolamine = S-adenosyl-L-homocysteine + N-methylphenylethanolamine random binding mechanism with a kinetically preferred order of binding where S-adenosyl-L-methionine is the preferred first substrate Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.28 adrenal medulla
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.28 normetanephrine + S-adenosyl-L-methionine
-
 Bos taurus metanephrine + S-adenosyl-L-homocysteine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.28 additional information
-
 presence of 2.5 mM normetanephrine has no effect on the heat denaturation, 1 mM S-adenosyl-L-methionine decreases the rate of inactivation Bos taurus
2.1.1.28 39
-
 stable for at least 50 min Bos taurus
2.1.1.28 45
-
 activity is rapidly destroyed Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.28 7.9
-
 Tris or borate buffer Bos taurus