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Literature summary extracted from

  • Dey, N.B.; Bounelis, P.; Fritz, T.A.; Bedwell, D.M.; Marchase, R.B.
    The glycosylation of phosphoglucomutase is modulated by carbon source and heat shock in Saccharomyces cerevisiae (1994), J. Biol. Chem., 269, 27143-27148.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.8.19 active fractions are frozen in a dry ice/methanol bath immediately after preparation and stored at -80°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.19 Ca2+ added in the activity assay at a final concentration of 5 mM Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.19 UDP-glucose + phosphoglucomutase Saccharomyces cerevisiae underglucosylated phosphoglucomutase increases GlcPTase activity in vitro, this underglucosylated state is maximized when galactose is used as carbon source or following a heat shock in Saccharomyces cerevisiae cultures ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.19 Saccharomyces cerevisiae
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 strains NY13 and YDB135
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.19 UDP-glucose + glycoprotein D-mannose
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 Saccharomyces cerevisiae UMP + glycoprotein 6-(D-glucose-1-phospho)-D-mannose
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 ?
2.7.8.19 UDP-glucose + phosphoglucomutase specific for UDP-glucose, the site affected in the GlcPTase reaction is not the enzymatic active site of phosphoglucomutase, underglucosylated forms of phosphoglucomutase obtained in response to heat shock and growth in media containing carbon sources other than glucose increase transfer in vitro GlcPTase assays and appear to reflect regulation of the GlcPTase activity Saccharomyces cerevisiae ?
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 ?
2.7.8.19 UDP-glucose + phosphoglucomutase underglucosylated phosphoglucomutase increases GlcPTase activity in vitro, this underglucosylated state is maximized when galactose is used as carbon source or following a heat shock in Saccharomyces cerevisiae cultures Saccharomyces cerevisiae ?
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 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.19 6
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 assay at, 50 mM Bis-Tris buffer Saccharomyces cerevisiae