Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Simianu, M.; Murakami, E.; Brewer, J.M.; Ragsdale, S.W.
    Purification and properties of the heme- and iron-sulfur-containing heterodisulfide reductase from Methanosarcina thermophila (1998), Biochemistry, 37, 10027-10039.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.98.1 membrane
-
Methanosarcina thermophila 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.98.1 Iron the enzyme contains two distinct [Fe4S4]2+/1+ clusters in the large subunit Methanosarcina thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.98.1 27000
-
x * 53000 + x * 27000, SDS-PAGE Methanosarcina thermophila
1.8.98.1 53000
-
x * 53000 + x * 27000, SDS-PAGE Methanosarcina thermophila
1.8.98.1 155000
-
gel filtration Methanosarcina thermophila
1.8.98.1 206000
-
equilibrium sedimentation Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.98.1 coenzyme B + coenzyme M + methanophenazine Methanosarcina thermophila
-
N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.8.98.1 Methanosarcina thermophila
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.8.98.1 lipoprotein
-
Methanosarcina thermophila

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.98.1
-
Methanosarcina thermophila

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.98.1 culture condition:acetate-grown cell
-
Methanosarcina thermophila
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.98.1 200
-
-
Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.98.1 coenzyme B + coenzyme M + methanophenazine
-
Methanosarcina thermophila N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + dihydromethanophenazine
-
?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylviologen
-
Methanosarcina thermophila coenzyme B + coenzyme M + methylviologen
-
?

Subunits

EC Number Subunits Comment Organism
1.8.98.1 ? x * 53000 + x * 27000, SDS-PAGE Methanosarcina thermophila

Synonyms

EC Number Synonyms Comment Organism
1.8.98.1 HDR
-
Methanosarcina thermophila

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.98.1 heme the enzyme contains two discrete b-type hemes in the small subunit. One heme is high-spin and has a high midpoint potential, -23 mV, whereas the other heme is low-spin and exhibits a relatively low midpoint potential, -180 mV Methanosarcina thermophila