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Literature summary extracted from

  • Genet, R.; Benetti, P.H.; Hammadi, A.; Menez, A.
    L-tryptophan 2',3'-oxidase from Chromobacterium violaceum (1995), J. Biol. Chem., 270, 23540-23545.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.3.10 active with 0.2 M dithiothreitol, 1% SDS, or 4.5 M urea Chromobacterium violaceum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.10 5-hydroxy-L-tryptophan competitive inhibition, Ki: 0.037 mM Chromobacterium violaceum
1.3.3.10 D-tryptophan competitive inhibition, Ki: 0.0066 mM Chromobacterium violaceum
1.3.3.10 methyl-3-indole competitive inhibition, Ki: 0.0013 mM Chromobacterium violaceum
1.3.3.10 additional information no inhibition with L-histidine, N-acetyl-L-phenylalaninamide and N-acetyl-L-tyrosinamide Chromobacterium violaceum
1.3.3.10 tryptamine competitive inhibition, Ki: 0.0054 mMl Chromobacterium violaceum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.10 0.0058
-
 L-tryptophan
-
 Chromobacterium violaceum
1.3.3.10 0.0091
-
 L-Tryptophanamide
-
 Chromobacterium violaceum
1.3.3.10 0.0195
-
 N-Acetyl-L-tryptophanamide
-
 Chromobacterium violaceum
1.3.3.10 0.02
-
 indole-3-propionate
-
 Chromobacterium violaceum
1.3.3.10 0.026
-
 pentagastrin dehydrogenation of the tryptophanyl side chain Chromobacterium violaceum
1.3.3.10 0.071
-
 Luteinizing hormone-releasing hormone dehydrogenation of the tryptophanyl side chain Chromobacterium violaceum
1.3.3.10 0.0791
-
 N-acetyl-L-tryptophan
-
 Chromobacterium violaceum
1.3.3.10 0.93
-
 adrenocorticotropic hormone dehydrogenation of the tryptophanyl side chain Chromobacterium violaceum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.10 MgCl2 10 mM, residual activity 103% Chromobacterium violaceum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.10 14000
-
 alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE Chromobacterium violaceum
1.3.3.10 74000
-
 alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE Chromobacterium violaceum
1.3.3.10 680000
-
 gel filtration Chromobacterium violaceum

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.10 Chromobacterium violaceum
-
 strain ATCC12472
-
1.3.3.10 Chromobacterium violaceum ATCC 12472
-
 strain ATCC12472
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.10 30.3
-
 after purification Chromobacterium violaceum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.10 adrenocorticotropic hormone + O2
-
 Chromobacterium violaceum ? + H2O2
-
 ?
1.3.3.10 indole-3-propionate + O2
-
 Chromobacterium violaceum ? + H2O2
-
 ?
1.3.3.10 indole-3-propionate + O2
-
 Chromobacterium violaceum ATCC 12472 ? + H2O2
-
 ?
1.3.3.10 L-tryptophan + O2
-
 Chromobacterium violaceum alpha,beta-didehydrotryptophan + H2O2
-
 ?
1.3.3.10 L-tryptophan + O2
-
 Chromobacterium violaceum ATCC 12472 alpha,beta-didehydrotryptophan + H2O2
-
 ?
1.3.3.10 L-tryptophanamide + O2
-
 Chromobacterium violaceum alpha,beta-didehydrotryptophanamide + H2O2
-
 ?
1.3.3.10 L-tryptophanamide + O2
-
 Chromobacterium violaceum ATCC 12472 alpha,beta-didehydrotryptophanamide + H2O2
-
 ?
1.3.3.10 luteinizing hormone-releasing hormone + O2
-
 Chromobacterium violaceum ? + H2O2
-
 ?
1.3.3.10 N-acetyl-L-tryptophan + O2
-
 Chromobacterium violaceum N-acetyl-alpha,beta-didehydrotryptophan + H2O2
-
 ?
1.3.3.10 N-acetyl-L-tryptophan + O2
-
 Chromobacterium violaceum ATCC 12472 N-acetyl-alpha,beta-didehydrotryptophan + H2O2
-
 ?
1.3.3.10 N-acetyl-L-tryptophanamide + O2
-
 Chromobacterium violaceum N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2
-
 ?
1.3.3.10 N-acetyl-L-tryptophanamide + O2
-
 Chromobacterium violaceum ATCC 12472 N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2
-
 ?
1.3.3.10 pentagastrin + O2
-
 Chromobacterium violaceum N-[(2E)-2-{[N-(tert-butoxycarbonyl)-beta-alanyl]amino}-3-(1H-indol-3-yl)prop-2-enoyl]-L-methionyl-L-alpha-aspartyl-L-phenylalaninamide + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.10 octamer alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE Chromobacterium violaceum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.3.10 80
-
 active up to 80øC Chromobacterium violaceum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.10 0.283
-
 adrenocorticotropic hormone dehydrogenation of the tryptophanyl side chain Chromobacterium violaceum
1.3.3.10 0.333
-
 Luteinizing hormone-releasing hormone dehydrogenation of the tryptophanyl side chain Chromobacterium violaceum
1.3.3.10 0.533
-
 pentagastrin dehydrogenation of the tryptophanyl side chain Chromobacterium violaceum
1.3.3.10 0.593
-
 L-Tryptophanamide
-
 Chromobacterium violaceum
1.3.3.10 0.66
-
 L-tryptophan
-
 Chromobacterium violaceum
1.3.3.10 0.753
-
 N-Acetyl-L-tryptophanamide
-
 Chromobacterium violaceum
1.3.3.10 0.785
-
 indole-3-propionate
-
 Chromobacterium violaceum
1.3.3.10 0.79
-
 N-acetyl-L-tryptophan
-
 Chromobacterium violaceum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.10 5
-
-
 Chromobacterium violaceum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.3.10 7
-
 after dilution in 0.1 M bis-Tris buffer Chromobacterium violaceum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.3.10 0.0013
-
 methyl-3-indole
-
 Chromobacterium violaceum
1.3.3.10 0.0054
-
 tryptamine
-
 Chromobacterium violaceum
1.3.3.10 0.0066
-
 D-tryptophan
-
 Chromobacterium violaceum
1.3.3.10 0.037
-
 5-hydroxy-L-tryptophan
-
 Chromobacterium violaceum