Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kolossov, V.L.; Rebeiz, C.A.
    Chloroplast biogenesis 84: solubilization and partial purification of membrane-bound [4-vinyl]chlorophyllide a reductase from etiolated barley leaves (2001), Anal. Biochem., 295, 214-219.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.1.75 glycerol stabilizes the membrane-bound enzyme, it has no effect on the solubilized enzyme Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.75 chloroplast
-
 Hordeum vulgare 9507
-
1.3.1.75 chloroplast
-
 Cucumis sativus 9507
-
1.3.1.75 etioplast
-
 Hordeum vulgare 9513
-
1.3.1.75 etioplast
-
 Cucumis sativus 9513
-
1.3.1.75 membrane
-
 Hordeum vulgare 16020
-
1.3.1.75 membrane
-
 Cucumis sativus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+ Hordeum vulgare key enzyme of the chlorophyll biosynthetic pathway 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+ Cucumis sativus key enzyme of the chlorophyll biosynthetic pathway 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.75 Cucumis sativus
-
 var. Poinsett 76
-
1.3.1.75 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.75 partial Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.75 leaf etiolated Hordeum vulgare
-
1.3.1.75 leaf etiolated Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.75 0.17
-
 partially purified enzyme Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
1.3.1.75 -80 °C, partially purified enzyme, stable for several months Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+
-
 Hordeum vulgare 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+
-
 Cucumis sativus 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+ key enzyme of the chlorophyll biosynthetic pathway Hordeum vulgare 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
1.3.1.75 2,4-divinyl chlorophyllide a + NADPH + H+ key enzyme of the chlorophyll biosynthetic pathway Cucumis sativus 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.75 25
-
 assay at Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.75 6.3
-
-
 Cucumis sativus
1.3.1.75 6.3
-
 assay at Hordeum vulgare