EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.7 | thiamine diphosphate | requires for maximal activity of enzyme | Pyrococcus furiosus | |
1.2.7.7 | thiamine diphosphate | requires for maximal activity of enzyme | Thermococcus litoralis | |
1.2.7.8 | thiamine diphosphate | - |
Pyrococcus furiosus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.7.7 | - |
Pyrococcus furiosus |
1.2.7.8 | - |
Pyrococcus furiosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.7 | additional information | - |
additional information | - |
Pyrococcus furiosus | |
1.2.7.7 | additional information | - |
additional information | - |
Thermococcus litoralis | |
1.2.7.8 | 0.017 | - |
CoA | - |
Pyrococcus furiosus | |
1.2.7.8 | 0.048 | - |
Ferredoxin | - |
Pyrococcus furiosus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.7 | Fe | each of the tetramer contains three [4Fe-4S] cluster | Pyrococcus furiosus | |
1.2.7.7 | Fe | each of the tetramer contains three [4Fe-4S] cluster | Thermococcus litoralis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.7 | 13000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
1.2.7.7 | 23000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
1.2.7.7 | 34000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
1.2.7.7 | 47000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
1.2.7.8 | 23000 | - |
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
1.2.7.8 | 24000 | - |
2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
1.2.7.8 | 66000 | - |
2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
1.2.7.8 | 71000 | - |
2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.7 | additional information | Pyrococcus furiosus | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | ? | - |
? | |
1.2.7.7 | additional information | Thermococcus litoralis | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | ? | - |
? | |
1.2.7.8 | (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Methanothermobacter thermautotrophicus | - |
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.8 | (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Pyrococcus furiosus | - |
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.7 | Pyrococcus furiosus | - |
- |
- |
1.2.7.7 | Thermococcus litoralis | - |
- |
- |
1.2.7.8 | Methanothermobacter thermautotrophicus | - |
- |
- |
1.2.7.8 | Pyrococcus furiosus | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.2.7.7 | enzyme is irreversibly inactivated by oxygen | Pyrococcus furiosus |
1.2.7.8 | irreversibly inactivated by oxygen | Pyrococcus furiosus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.7 | under anaerobic conditions | Pyrococcus furiosus |
1.2.7.8 | - |
Pyrococcus furiosus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | pathway | Pyrococcus furiosus | |
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | pathway | Thermococcus litoralis | |
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Pyrococcus furiosus | |
1.2.7.7 | 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Thermococcus litoralis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.7 | 2-ketobutyrate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | propanoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | 2-ketobutyrate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | propanoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | 2-ketoisocaproate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | 2-ketoisocaproate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | 2-ketoisovalerate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | 2-ketoisovalerate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
1.2.7.7 | 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | 4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | indolpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | indolacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | indolpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | indolacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | additional information | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | Pyrococcus furiosus | ? | - |
? | |
1.2.7.7 | additional information | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | Thermococcus litoralis | ? | - |
? | |
1.2.7.7 | additional information | enzyme catalyses also the nonoxidative decarboxylation of pyruvate to acetaldehyde | Pyrococcus furiosus | ? | - |
? | |
1.2.7.7 | additional information | enzyme catalyses also the nonoxidative decarboxylation of pyruvate to acetaldehyde | Thermococcus litoralis | ? | - |
? | |
1.2.7.7 | additional information | no substrate is 2-ketoglutarate | Pyrococcus furiosus | ? | - |
? | |
1.2.7.7 | additional information | no substrate is 2-ketoglutarate | Thermococcus litoralis | ? | - |
? | |
1.2.7.7 | phenylglyoxylate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | benzoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | phenylglyoxylate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | benzoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | phenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | phenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.7 | pyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.8 | (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Methanothermobacter thermautotrophicus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.8 | (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.8 | (indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-2-(indol-3-yl)acetyl-CoA + CO2 + 2 reduced ferredoxin + H+ | - |
r | |
1.2.7.8 | 2-keto-4-methylthiobutyrate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | CO2 + reduced ferredoxin + 3-methylthiopropionyl-CoA | - |
r | |
1.2.7.8 | 2-ketoisocaproate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | CO2 + reduced ferredoxin + isovaleryl-CoA | - |
r | |
1.2.7.8 | indolepyruvate + CoA + oxidized methylviologen | - |
Pyrococcus furiosus | indoleacetyl-CoA + CO2 + reduced methylviologen | - |
? | |
1.2.7.8 | additional information | pyruvate, 2-ketoisovalerate, 2-ketoglutarate, 2 ketobutyrate, and phenylglyoxylate are no substrates | Pyrococcus furiosus | ? | - |
? | |
1.2.7.8 | p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.8 | phenylpyruvate + CoA + oxidized ferredoxin | - |
Methanothermobacter thermautotrophicus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
1.2.7.8 | phenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.7.7 | octamer | alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
1.2.7.8 | tetramer | - |
Methanothermobacter thermautotrophicus |
1.2.7.8 | tetramer | 2 * 66000 + 2 * 23000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
1.2.7.8 | tetramer | 2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |