BRENDA - Enzyme Database

Mouse liver cytidine-5-monophosphate-N-acetylneuraminic acid hydroxylase. Catalytic function and regulation

Shaw, L.; Schneckenburger, P.; Carlsen, J.; Christiansen, K.; Schauer, R.; Eur. J. Biochem. 206, 269-277 (1992)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.18.2
decyl glucoside
activation
Mus musculus
1.14.18.2
additional information
highest activity in 50 mM Hepes buffer, significant inhibition at increasing concentrations
Mus musculus
1.14.18.2
Nonidet P-40
effective inhibitor
Mus musculus
1.14.18.2
Octanoic acid
1 mM, modest activation
Mus musculus
1.14.18.2
octyl glucoside
effective inhibitor
Mus musculus
1.14.18.2
SDS
1 mM, modest activation
Mus musculus
1.14.18.2
Triton X-100
effective inhibitor
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.2
1,10-phenanthroline
5 mM, complete inhibition
Mus musculus
1.14.18.2
anti-(rat cytochrome b5) antiserum
-
Mus musculus
1.14.18.2
cardiolipin
3 mM, 15% inhibition
Mus musculus
1.14.18.2
CMP-N-glycoloylneuraminate
-
Mus musculus
1.14.18.2
ferrozine
0.7 mM, 82% inhibition
Mus musculus
1.14.18.2
KCN
5 mM, 49% inhibition
Mus musculus
1.14.18.2
Na2HPO4
2 mM, 86% inhibition
Mus musculus
1.14.18.2
Na4P2O7
2 mM, 54% loss of activity
Mus musculus
1.14.18.2
phosphatidic acid
3 mM, 20% inhibition
Mus musculus
1.14.18.2
phosphatidylinositol
3 mM, 68% inhibition
Mus musculus
1.14.18.2
Tiron
5 mM, 82% inhibition
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.2
17000
-
gel filtration
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.18.2
Mus musculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.2
liver
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.2
CMP-N-acetylneuraminate + ferrocytochrome b5 + O2
the electron carrier cytochrome b5 is essential for activity
639334
Mus musculus
CMP-N-glycoloylneuraminate + ferricytrochrome b5 + H2O
-
-
-
?
1.14.18.2
CMP-N-acetylneuraminate + NADH + O2
-
639334
Mus musculus
CMP-N-glycoloylneuraminate + NAD+ + H2O
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.2
6.8
7.4
-
Mus musculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.18.2
ferrocytochrome b5
the electron carrier cytochrome b5 is essential for activity
Mus musculus
1.14.18.2
NADH
-
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.18.2
decyl glucoside
activation
Mus musculus
1.14.18.2
additional information
highest activity in 50 mM Hepes buffer, significant inhibition at increasing concentrations
Mus musculus
1.14.18.2
Nonidet P-40
effective inhibitor
Mus musculus
1.14.18.2
Octanoic acid
1 mM, modest activation
Mus musculus
1.14.18.2
octyl glucoside
effective inhibitor
Mus musculus
1.14.18.2
SDS
1 mM, modest activation
Mus musculus
1.14.18.2
Triton X-100
effective inhibitor
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.18.2
ferrocytochrome b5
the electron carrier cytochrome b5 is essential for activity
Mus musculus
1.14.18.2
NADH
-
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.2
1,10-phenanthroline
5 mM, complete inhibition
Mus musculus
1.14.18.2
anti-(rat cytochrome b5) antiserum
-
Mus musculus
1.14.18.2
cardiolipin
3 mM, 15% inhibition
Mus musculus
1.14.18.2
CMP-N-glycoloylneuraminate
-
Mus musculus
1.14.18.2
ferrozine
0.7 mM, 82% inhibition
Mus musculus
1.14.18.2
KCN
5 mM, 49% inhibition
Mus musculus
1.14.18.2
Na2HPO4
2 mM, 86% inhibition
Mus musculus
1.14.18.2
Na4P2O7
2 mM, 54% loss of activity
Mus musculus
1.14.18.2
phosphatidic acid
3 mM, 20% inhibition
Mus musculus
1.14.18.2
phosphatidylinositol
3 mM, 68% inhibition
Mus musculus
1.14.18.2
Tiron
5 mM, 82% inhibition
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.2
17000
-
gel filtration
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.2
liver
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.2
CMP-N-acetylneuraminate + ferrocytochrome b5 + O2
the electron carrier cytochrome b5 is essential for activity
639334
Mus musculus
CMP-N-glycoloylneuraminate + ferricytrochrome b5 + H2O
-
-
-
?
1.14.18.2
CMP-N-acetylneuraminate + NADH + O2
-
639334
Mus musculus
CMP-N-glycoloylneuraminate + NAD+ + H2O
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.2
6.8
7.4
-
Mus musculus