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Literature summary extracted from

  • Schlenzka, W.; Shaw, L.; Schneckenburger, P.; Schauer, R.
    Purification and characterization of CMP-N-acetylneuraminic acid hydroxylase from pig submandibular glands (1994), Glycobiology, 4, 675-683.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.2 1,10-phenanthroline
-
 Sus scrofa
1.14.18.2 ferrozine
-
 Sus scrofa
1.14.18.2 KCN
-
 Sus scrofa
1.14.18.2 Tiron
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.2 0.003
-
 CMP-N-acetylneuraminate pH 7.4, 37°C, reconstituted system consisting of purified hydroxylase, cytochrome b5, cytochrome b5 reductase and catalase Sus scrofa
1.14.18.2 0.011
-
 CMP-N-acetylneuraminate pH 7.4, 37°C, purified hydroxylase in presence of Triton X-100 solubilized microsomes Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.2 60000
-
 gel filtration Sus scrofa
1.14.18.2 65000
-
 1 * 65000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.2
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.2 submandibular gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.2 0.816
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.2 CMP-N-acetylneuraminate + NADH + O2
-
 Sus scrofa CMP-N-glycoloylneuraminate + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.2 monomer 1 * 65000, SDS-PAGE Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.2 NADH
-
 Sus scrofa