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Literature summary extracted from

  • Vioque, B.; Castellano, J.M.
    Extraction and biochemical characterization of 1-aminocyclopropane-1-carboxylic acid oxidase from pear (1994), Physiol. Plant., 90, 334-338.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.17.4 ascorbate absolutely dependent on Pyrus communis
1.14.17.4 CO2 absolutely dependent on Pyrus communis
1.14.17.4 CO2 best at 14% or higher Pyrus communis
1.14.17.4 O2 dependent on Pyrus communis
1.14.17.4 Triton X-100 best at 0.8% or above Pyrus communis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.4 alpha-aminoisobutyric acid
-
 Pyrus communis
1.14.17.4 Co2+
-
 Pyrus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.4 0.057
-
 1-aminocyclopropane-1-carboxylate pH 6.7, 30°C Pyrus communis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.4 Fe2+ absolutely required Pyrus communis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 Pyrus communis
-
 ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.4 Pyrus communis
-
 cv.Blanquilla
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.17.4 fruit
-
 Pyrus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.4 0.0047
-
 crude enzyme extract Pyrus communis
1.14.17.4 0.006
-
 pear fruit prior homogenization Pyrus communis

Storage Stability

EC Number Storage Stability Organism
1.14.17.4 -20°C, 30% glycerol, stable for 11 days Pyrus communis
1.14.17.4 2°C, 30% glycerol, loss of 30% activity after 24 h Pyrus communis
1.14.17.4 2°C, 30% glycerol, loss of 85% activity after 7 h Pyrus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2
-
 Pyrus communis ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 absolutely dependent on CO2 Pyrus communis ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 i.e. ACC Pyrus communis ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 absolutely dependent on ascorbate Pyrus communis ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.4 28
-
 2 optima at 28°C and 38°C Pyrus communis
1.14.17.4 38
-
 2 optima at 28°C and 38°C Pyrus communis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.17.4 4.2
-
 alpha-aminoisobutyric acid pH 6.7, 30°C Pyrus communis