Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Charng, Y.; Chou, S.J.; Jiaang, W.T.; Chen, S.T.; Yang, S.F.
    The catalytic mechanism of 1-aminocyclopropane-1-carboxylic acid oxidase (2001), Arch. Biochem. Biophys., 385, 179-185.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.4 expressed in Escherichia coli Malus domestica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.4 alpha-aminoisobutyric acid competitive to 1-aminocyclopropane-1-carboxylate Malus domestica
1.14.17.4 D-alanine competitive to 1-aminocyclopropane-1-carboxylate Malus domestica
1.14.17.4 D-alpha-aminobutyric acid competitive to 1-aminocyclopropane-1-carboxylate Malus domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.4 0.23
-
 1-aminocyclopropane-1-carboxylate recombinant enzyme, pH 7.0, 30°C Malus domestica
1.14.17.4 14.7
-
 alpha-aminoisobutyrate recombinant enzyme, pH 7.0, 30°C Malus domestica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 Malus domestica
-
 ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.4 Malus domestica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.4 recombinant from Escherichia coli Malus domestica

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethene + cyanide + dehydroascorbate + CO2 + 2 H2O catalytic mechanism Malus domestica
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.17.4 0.003
-
 at 10 mM L-alpha-aminobutyric acid, purified recombinant enzyme Malus domestica
1.14.17.4 0.008
-
 at 10 mM L-alanine, purified recombinant enzyme Malus domestica
1.14.17.4 0.023
-
 at 10 mM D-alanine, purified recombinant enzyme Malus domestica
1.14.17.4 0.051
-
 at 10 mM alpha-aminoisobutyric acid, purified recombinant enzyme Malus domestica
1.14.17.4 0.059
-
 at 10 mM D-alpha-aminobutyric acid, purified recombinant enzyme Malus domestica
1.14.17.4 0.13
-
 at 10 mM 1-aminocyclopropane-1-carboxylate, purified recombinant enzyme Malus domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2
-
 Malus domestica ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 stereoselectivity Malus domestica ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 i.e. ACC Malus domestica ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 N-hydroxyl-1-aminocyclopropane-1-carboxylate is no intermediate Malus domestica ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 alpha-aminoisobutyric acid + L-ascorbate + O2
-
 Malus domestica ammonia + acetone + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 D-alanine + L-ascorbate + O2
-
 Malus domestica ?
-
 ?
1.14.17.4 D-alpha-aminobutyric acid + L-ascorbate + O2
-
 Malus domestica ammonia + acetone + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 L-alanine + L-ascorbate + O2
-
 Malus domestica ?
-
 ?
1.14.17.4 L-alpha-aminobutyric acid + L-ascorbate + O2
-
 Malus domestica ammonia + acetone + dehydroascorbate + CO2 + H2O
-
 ?
1.14.17.4 N-hydroxy-alpha-aminoisobutyric acid + L-ascorbate + O2
-
 Malus domestica ammonia + acetone + dehydroascorbate + CO2 + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.17.4 30
-
 assay at Malus domestica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.17.4 0.07
-
 1-aminocyclopropane-1-carboxylate recombinant enzyme, pH 7.0, 30°C Malus domestica
1.14.17.4 0.07
-
 alpha-aminoisobutyrate recombinant enzyme, pH 7.0, 30°C Malus domestica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.17.4 7
-
 assay at Malus domestica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.17.4 4.2
-
 D-alpha-aminobutyric acid pH 7.0, 30°C Malus domestica
1.14.17.4 7.5
-
 alpha-aminoisobutyric acid pH 7.0, 30°C Malus domestica
1.14.17.4 28
-
 D-alanine pH7.0, 30°C Malus domestica