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Literature summary extracted from
- In vivo and in vitro 1-aminocyclopropane-1-carboxylic acid oxidase activity in pear fruit: role of ascorbate and inactivation during catalysis (1998), J. Agric. Food Chem., 46, 1706-1711.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.4 | ascorbate | cannot be replaced by glutathione and NADPH | Pyrus communis |  |
| 1.14.17.4 | ascorbate | required invitro, inhibitory in vivo | Pyrus communis | |
| 1.14.17.4 | CO2 | activates | Pyrus communis | |
| 1.14.17.4 | CO2 | required | Pyrus communis | |
| 1.14.17.4 | dithiothreitol | activates in presence of ascorbate, but cannot replace ascorbate | Pyrus communis | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.17.4 | highly unstable under assay conditions | Pyrus communis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.4 | 2-oxoglutarate | at 1 mM | Pyrus communis | |
| 1.14.17.4 | ascorbate | required in vitro, inhibitory in vivo | Pyrus communis | |
| 1.14.17.4 | catalase | - |
Pyrus communis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.17.4 | Fe2+ | - |
Pyrus communis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.17.4 | 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Pyrus communis | catalyses the final step in ethylene biosynthesis | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.17.4 | Pyrus communis | - |
cv.Blanquilla | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.17.4 | partial | Pyrus communis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.17.4 | fruit | - |
Pyrus communis | - |
| 1.14.17.4 | pericarp | - |
Pyrus communis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.17.4 | 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | catalyses the final step in ethylene biosynthesis | Pyrus communis | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| 1.14.17.4 | 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | specific for 1-aminocyclopropane-1-carboxylate | Pyrus communis | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
| 1.14.17.4 | 1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Pyrus communis | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.4 | 28 | - |
assay at | Pyrus communis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.17.4 | 6.7 | - |
assay at | Pyrus communis |
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