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Literature summary extracted from

  • Bhat, S.G.; Vaidyanathan, C.S.
    Purifications and properties of L-mandelate-4-hydroxylase from Pseudomonas convexa (1976), Arch. Biochem. Biophys., 176, 314-323.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.6 2-amino-4-hydroxy-6,7-dimethyl-tetrahydropteridine pteridine cofactor required, 2-amino-4-hydroxy-6,7-dimethyl-tetrahydropteridine is most effective, optimal concentration is 0.000025 mM Pseudomonas putida
1.14.16.6 tetrahydrofolic acid pteridine cofactor required, 50% of the activity of 2-amino-4-hydroxy-6,7-dimethyl-tetrahydropteridine Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.6 1,10-phenanthroline 0.5 mM, 88% inhibition Pseudomonas putida
1.14.16.6 2,2'-bipyridyl 0.5 mM, 83% inhibition Pseudomonas putida
1.14.16.6 2-amino-4-hydroxy-6,7-dimethyl-tetrahydropteridine above 0.000025 mM, slight inhibition Pseudomonas putida
1.14.16.6 4-hydroxymercuribenzoate partially reversible by thiol compounds Pseudomonas putida
1.14.16.6 8-hydroxyquinoline 0.5 mM, 60% inhibition Pseudomonas putida
1.14.16.6 Ag2+ 0.0001 mM, 42% inhibition Pseudomonas putida
1.14.16.6 amethopterin
-
 Pseudomonas putida
1.14.16.6 Aminopterine
-
 Pseudomonas putida
1.14.16.6 Cd2+ 0.0001 mM, 66% inhibition Pseudomonas putida
1.14.16.6 Cu2+ 0.0001 mM, 40% inhibition Pseudomonas putida
1.14.16.6 diethyldithiocarbamate 0.5 mM, 16% inhibition Pseudomonas putida
1.14.16.6 EDTA 0.5 mM, 64% inhibition Pseudomonas putida
1.14.16.6 guanidine hydrochloride 2.0 mM, 95% inhibition Pseudomonas putida
1.14.16.6 Hg2+ 0.0001 mM, 56% inhibition Pseudomonas putida
1.14.16.6 iodoacetamide weak Pseudomonas putida
1.14.16.6 iodoacetate weak Pseudomonas putida
1.14.16.6 NEM
-
 Pseudomonas putida
1.14.16.6 SDS 0.1 mM, complete inhibition Pseudomonas putida
1.14.16.6 Thiourea 1.0 mM, complete inhibition Pseudomonas putida
1.14.16.6 Urea 2.0 mM, complete inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.6 0.1
-
 (S)-2-Hydroxy-2-phenylacetate
-
 Pseudomonas putida
1.14.16.6 0.19
-
 NADPH
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.6 Fe2+ required Pseudomonas putida
1.14.16.6 Fe2+ Km: 0.047 mM Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.6 91000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.6 (S)-2-hydroxy-2-phenylacetate + tetrahydropteridine + O2 Pseudomonas putida i.e. L-mandelate, first step of oxidative degradation of L-mandelate by Pseudomonas convexa ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.6 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.6
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.6 0.021
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.14.16.6 -20°C, partially purified enzyme is stable for 36-40 h Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.6 (S)-2-hydroxy-2-phenylacetate + tetrahydropteridine i.e. L-mandelate, highly substrate specific, D-mandelate is not hydroxylated Pseudomonas putida (S)-4-hydroxymandelate + dihydropteridine + H2O
-
 ?
1.14.16.6 (S)-2-hydroxy-2-phenylacetate + tetrahydropteridine + O2 i.e. L-mandelate, first step of oxidative degradation of L-mandelate by Pseudomonas convexa Pseudomonas putida ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.16.6 38
-
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.16.6 55
-
 5 min, no loss of activity Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.16.6 5.4
-
 citrate-phosphate buffer Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.16.6 4.6 7.4 half-maximal activity at pH 4.6 and pH 7.4 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.6 NADPH required Pseudomonas putida