EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.82 | expression in Escherichia coli | Acinetobacter sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.82 | H156R | substituting an iron-binding ligand | Acinetobacter sp. |
1.14.13.82 | additional information | several amino acid substitutions give indications of the extent to which amino acid substitutions can be tolerated at specified positions | Acinetobacter sp. |
1.14.13.82 | W217R | functional at 22øC but not at 37øC | Acinetobacter sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.82 | 4-hydroxy-3,5-dimethylbenzoate | 20% competitive inhibition at 3 mM | Acinetobacter sp. | |
1.14.13.82 | m-anisate | 15-77.5% competitive inhibition of vanillate demethylation between 1-3 mM | Acinetobacter sp. | |
1.14.13.82 | m-toluate | 45% competitive inhibition at 3 mM | Acinetobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.82 | vanillate + O2 + NADH + H+ | Acinetobacter sp. | able to demethylate one methoxy group or to monohydroxylate one methyl group in the meta position | 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.82 | Acinetobacter sp. | - |
strain ADP1 and ADP230 | - |
1.14.13.82 | Acinetobacter sp. ADP1 | - |
strain ADP1 and ADP230 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.82 | cell culture | - |
Acinetobacter sp. | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.82 | 3,4,5-trimethoxybenzoate + O2 + NADH + H+ | 70% of the activity compared to vanillate | Acinetobacter sp. | 3-hydroxy-4,5-dimethoxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
1.14.13.82 | 3,4,5-trimethoxybenzoate + O2 + NADH + H+ | 70% of the activity compared to vanillate | Acinetobacter sp. ADP1 | 3-hydroxy-4,5-dimethoxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
1.14.13.82 | 3,4-dimethoxybenzoate + O2 + NADH + H+ | - |
Acinetobacter sp. | isovanillate + NAD+ + H2O + formaldehyde | - |
? | |
1.14.13.82 | 3,4-dimethoxybenzoate + O2 + NADH + H+ | - |
Acinetobacter sp. ADP1 | isovanillate + NAD+ + H2O + formaldehyde | - |
? | |
1.14.13.82 | 3-(hydroxymethyl)-benzoate + NAD+ + H2O + formaldehyde | - |
Acinetobacter sp. | m-toluate + O2 + NADH + H+ | - |
? | |
1.14.13.82 | 3-(hydroxymethyl)-benzoate + NAD+ + H2O + formaldehyde | - |
Acinetobacter sp. ADP1 | m-toluate + O2 + NADH + H+ | - |
? | |
1.14.13.82 | 3-hydroxymethyl-4-hydroxy-5-methylbenzoate + NADH + H+ + ? | 85% of the activity compared to vanillate | Acinetobacter sp. | 4-hydroxy-3,5-dimethylbenzoate + NAD+ + ? | - |
? | |
1.14.13.82 | 3-hydroxymethyl-4-hydroxy-5-methylbenzoate + NADH + H+ + ? | 85% of the activity compared to vanillate | Acinetobacter sp. ADP1 | 4-hydroxy-3,5-dimethylbenzoate + NAD+ + ? | - |
? | |
1.14.13.82 | m-anisate + O2 + NADH + H+ | - |
Acinetobacter sp. | m-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
1.14.13.82 | m-anisate + O2 + NADH + H+ | - |
Acinetobacter sp. ADP1 | m-hydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
1.14.13.82 | vanillate + O2 + NADH + H+ | able to demethylate one methoxy group or to monohydroxylate one methyl group in the meta position | Acinetobacter sp. | 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde | - |
r |