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Literature summary extracted from

  • Morawski, B.; Segura, A.; Ornston, L.N.
    Substrate range and genetic analysis of Acinetobacter vanillate demethylase (2000), J. Bacteriol., 182, 1383-1389.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.82 expression in Escherichia coli Acinetobacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.82 H156R substituting an iron-binding ligand Acinetobacter sp.
1.14.13.82 additional information several amino acid substitutions give indications of the extent to which amino acid substitutions can be tolerated at specified positions Acinetobacter sp.
1.14.13.82 W217R functional at 22øC but not at 37øC Acinetobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.82 4-hydroxy-3,5-dimethylbenzoate 20% competitive inhibition at 3 mM Acinetobacter sp.
1.14.13.82 m-anisate 15-77.5% competitive inhibition of vanillate demethylation between 1-3 mM Acinetobacter sp.
1.14.13.82 m-toluate 45% competitive inhibition at 3 mM Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.82 vanillate + O2 + NADH + H+ Acinetobacter sp. able to demethylate one methoxy group or to monohydroxylate one methyl group in the meta position 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.82 Acinetobacter sp.
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strain ADP1 and ADP230
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1.14.13.82 Acinetobacter sp. ADP1
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strain ADP1 and ADP230
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.82 cell culture
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Acinetobacter sp.
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.82 3,4,5-trimethoxybenzoate + O2 + NADH + H+ 70% of the activity compared to vanillate Acinetobacter sp. 3-hydroxy-4,5-dimethoxybenzoate + NAD+ + H2O + formaldehyde
-
?
1.14.13.82 3,4,5-trimethoxybenzoate + O2 + NADH + H+ 70% of the activity compared to vanillate Acinetobacter sp. ADP1 3-hydroxy-4,5-dimethoxybenzoate + NAD+ + H2O + formaldehyde
-
?
1.14.13.82 3,4-dimethoxybenzoate + O2 + NADH + H+
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Acinetobacter sp. isovanillate + NAD+ + H2O + formaldehyde
-
?
1.14.13.82 3,4-dimethoxybenzoate + O2 + NADH + H+
-
Acinetobacter sp. ADP1 isovanillate + NAD+ + H2O + formaldehyde
-
?
1.14.13.82 3-(hydroxymethyl)-benzoate + NAD+ + H2O + formaldehyde
-
Acinetobacter sp. m-toluate + O2 + NADH + H+
-
?
1.14.13.82 3-(hydroxymethyl)-benzoate + NAD+ + H2O + formaldehyde
-
Acinetobacter sp. ADP1 m-toluate + O2 + NADH + H+
-
?
1.14.13.82 3-hydroxymethyl-4-hydroxy-5-methylbenzoate + NADH + H+ + ? 85% of the activity compared to vanillate Acinetobacter sp. 4-hydroxy-3,5-dimethylbenzoate + NAD+ + ?
-
?
1.14.13.82 3-hydroxymethyl-4-hydroxy-5-methylbenzoate + NADH + H+ + ? 85% of the activity compared to vanillate Acinetobacter sp. ADP1 4-hydroxy-3,5-dimethylbenzoate + NAD+ + ?
-
?
1.14.13.82 m-anisate + O2 + NADH + H+
-
Acinetobacter sp. m-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
?
1.14.13.82 m-anisate + O2 + NADH + H+
-
Acinetobacter sp. ADP1 m-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
?
1.14.13.82 vanillate + O2 + NADH + H+ able to demethylate one methoxy group or to monohydroxylate one methyl group in the meta position Acinetobacter sp. 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
r