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Literature summary extracted from

  • Doan, L.X.; Hassan, A.; Lipscomb, S.J.; Dhanda, A.; Zhang, Z.; Schofield, C.J.
    Mutagenesis studies on the iron binding ligands of clavaminic acid synthase (2000), Biochem. Biophys. Res. Commun., 279, 240-244.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.21 C162L isozyme CAS2, site-directed mutagenesis, same activity in hydroxylation of N-alpha-acetyl-L-arginine as the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 C200L isozyme CAS2, site-directed mutagenesis, 42% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 C201L isozyme CAS2, site-directed mutagenesis, 40% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 C8L isozyme CAS2, site-directed mutagenesis, 42% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 H109L isozyme CAS2, site-directed mutagenesis, 86% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 H122L isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 H131L isozyme CAS2, site-directed mutagenesis, 36% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 H145E isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 H145L isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 H145Q site-directed mutagenesis, no activity in hydroxylation of N-alpha-acetyl-L-arginine Streptomyces clavuligerus
1.14.11.21 H151l isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 H167L isozyme CAS2, site-directed mutagenesis, 19% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 H280L isozyme CAS2, site-directed mutagenesis, 86% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 H297L isozyme CAS2, site-directed mutagenesis, 3% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus
1.14.11.21 Q154L isozyme CAS2, site-directed mutagenesis, 23% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme Streptomyces clavuligerus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.21 Fe2+ required Streptomyces clavuligerus
1.14.11.21 Fe2+ H145 forms part of the HXE iron-binding motif in isozyme CAS2 Streptomyces clavuligerus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.21 Streptomyces clavuligerus
-
 isozyme CAS2
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.11.21 dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O reaction mechanism Streptomyces clavuligerus
a
1.14.11.21 proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O reaction mechanism Streptomyces clavuligerus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.21 deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 hydroxylation Streptomyces clavuligerus guanidinoproclavaminate + succinate + CO2 + H2O
-
 ?
1.14.11.21 dihydroclavaminate + 2-oxoglutarate + O2 cyclization Streptomyces clavuligerus clavaminate + succinate + CO2 + H2O
-
 ?
1.14.11.21 N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 hydroxylation Streptomyces clavuligerus 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O
-
 ?
1.14.11.21 N-alpha-acetyl-L-arginine + 2-oxoglutarate + O2 isozyme CAS2 Streptomyces clavuligerus 4-hydroxy-N-alpha-acetyl-L-arginine + succinate + CO2 + H2O
-
 ?
1.14.11.21 proclavaminate + 2-oxoglutarate + O2 saturation Streptomyces clavuligerus dihydroclavaminate + succinate + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.21 CAS
-
 Streptomyces clavuligerus