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Literature summary extracted from
- Purification and characterization of clavaminate synthase from Streptomyces antibioticus. A multifunctional enzyme of clavam biosynthesis (1995), J. Biol. Chem., 270, 5399-5404.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.21 | 0.0032 | - |
Fe2+ | pH 7.0, 22°C | Streptomyces antibioticus |  |
| 1.14.11.21 | 0.039 | - |
2-oxoglutarate | pH 7.0, 22°C | Streptomyces antibioticus | |
| 1.14.11.21 | 0.38 | - |
(2S,3R)-proclavaminate | pH 7.0, 22°C | Streptomyces antibioticus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.21 | Fe2+ | required | Streptomyces antibioticus | |
| 1.14.11.21 | Fe2+ | required | Streptomyces clavuligerus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.21 | 35360 | - |
CS1, mass spectrometry | Streptomyces clavuligerus |
| 1.14.11.21 | 35750 | - |
CS2, mass spectrometry | Streptomyces clavuligerus |
| 1.14.11.21 | 35840 | - |
CS3, mass spectrometry | Streptomyces antibioticus |
| 1.14.11.21 | 38000 | - |
CS1, gel filtration | Streptomyces clavuligerus |
| 1.14.11.21 | 41000 | - |
CS3, gel filtration | Streptomyces antibioticus |
| 1.14.11.21 | 42000 | - |
CS2, gel filtration | Streptomyces clavuligerus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.21 | deoxyamidinoproclavaminate + 2-oxoglutarate + O2 | Streptomyces antibioticus | - |
amidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | deoxyamidinoproclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
amidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | Streptomyces antibioticus | - |
guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | Streptomyces antibioticus Tu1718 | - |
guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | dihydroclavaminate + 2-oxoglutarate + O2 | Streptomyces antibioticus | - |
clavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | dihydroclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
clavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | additional information | Streptomyces antibioticus | enzyme plays a critical role in the biosynthesis of the clavam metabolites | ? | - |
? | |
| 1.14.11.21 | additional information | Streptomyces clavuligerus | enzyme plays a critical role in the biosynthesis of the clavam metabolites | ? | - |
? | |
| 1.14.11.21 | additional information | Streptomyces antibioticus | biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor | ? | - |
? | |
| 1.14.11.21 | additional information | Streptomyces clavuligerus | biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor | ? | - |
? | |
| 1.14.11.21 | additional information | Streptomyces antibioticus Tu1718 | enzyme plays a critical role in the biosynthesis of the clavam metabolites | ? | - |
? | |
| 1.14.11.21 | additional information | Streptomyces antibioticus Tu1718 | biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor | ? | - |
? | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | Streptomyces antibioticus | - |
dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.21 | Streptomyces antibioticus | - |
strain Tü 1718, CS3, no production of clavulanic acid | - |
| 1.14.11.21 | Streptomyces antibioticus Tu1718 | - |
strain Tü 1718, CS3, no production of clavulanic acid | - |
| 1.14.11.21 | Streptomyces clavuligerus | - |
2 isozymes CS1 and CS2 | - |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.14.11.21 | inactivation due to release of peroxide from reaction of 2-oxoglutarate and O2 in absence of proclavaminate, in presence of Fe2+, t1/2: 52 s, catalase partially protects | Streptomyces clavuligerus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.11.21 | 12.5fold to homogeneity | Streptomyces antibioticus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.11.21 | deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2 | reaction mechanism | Streptomyces antibioticus | |
| 1.14.11.21 | deoxyamidinoproclavaminate + 2-oxoglutarate + O2 = amidinoproclavaminate + succinate + CO2 | reaction mechanism | Streptomyces clavuligerus | |
| 1.14.11.21 | dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces antibioticus | |
| 1.14.11.21 | dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces antibioticus | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.21 | 0.1 | - |
purified enzyme | Streptomyces antibioticus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.11.21 | -80°C, purified protein, 50 mM MOPS, pH 7.0, 0.02 mM EDTA, 2 mM DTT, 50% glycerol, stable for at least 1 year | Streptomyces antibioticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.21 | (2S,3R)-proclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces antibioticus | clavaminate + succinate + CO2 + H2O | - |
r | |
| 1.14.11.21 | 2-oxoglutarate + O2 | side reaction in absence of proclavaminate, oxidative decarboxylation of 2-oxoglutarate in presence of Fe2+ and formation of peroxide, which inactivates the enzyme | Streptomyces antibioticus | ? + H2O2 | - |
? | |
| 1.14.11.21 | 2-oxoglutarate + O2 | side reaction in absence of proclavaminate, oxidative decarboxylation of 2-oxoglutarate in presence of Fe2+ and formation of peroxide, which inactivates the enzyme | Streptomyces clavuligerus | ? + H2O2 | - |
? | |
| 1.14.11.21 | deoxyamidinoproclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces antibioticus | amidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | deoxyamidinoproclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | amidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces antibioticus | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | hydroxylation | Streptomyces antibioticus | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | hydroxylation | Streptomyces clavuligerus | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces antibioticus Tu1718 | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | hydroxylation | Streptomyces antibioticus Tu1718 | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | dihydroclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces antibioticus | clavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | dihydroclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | dihydroclavaminate + 2-oxoglutarate + O2 | cyclization | Streptomyces antibioticus | clavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | dihydroclavaminate + 2-oxoglutarate + O2 | cyclization | Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | additional information | dependent on O2 and 2-oxoglutarate | Streptomyces antibioticus | ? | - |
? | |
| 1.14.11.21 | additional information | dependent on O2 and 2-oxoglutarate | Streptomyces clavuligerus | ? | - |
? | |
| 1.14.11.21 | additional information | enzyme plays a critical role in the biosynthesis of the clavam metabolites | Streptomyces antibioticus | ? | - |
? | |
| 1.14.11.21 | additional information | enzyme plays a critical role in the biosynthesis of the clavam metabolites | Streptomyces clavuligerus | ? | - |
? | |
| 1.14.11.21 | additional information | biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor | Streptomyces antibioticus | ? | - |
? | |
| 1.14.11.21 | additional information | biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor | Streptomyces clavuligerus | ? | - |
? | |
| 1.14.11.21 | additional information | dependent on O2 and 2-oxoglutarate | Streptomyces antibioticus Tu1718 | ? | - |
? | |
| 1.14.11.21 | additional information | enzyme plays a critical role in the biosynthesis of the clavam metabolites | Streptomyces antibioticus Tu1718 | ? | - |
? | |
| 1.14.11.21 | additional information | biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor | Streptomyces antibioticus Tu1718 | ? | - |
? | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces antibioticus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | saturation | Streptomyces antibioticus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | saturation | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | no activity with the racemic erythro isomers (2S,3S) and (2R,3R) | Streptomyces antibioticus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | no activity with the racemic erythro isomers (2S,3S) and (2R,3R) | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | specific for the threo-(2S,3R) enantiomer | Streptomyces antibioticus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
| 1.14.11.21 | proclavaminate + 2-oxoglutarate + O2 | specific for the threo-(2S,3R) enantiomer | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.11.21 | monomer | 1 * 35357-45000, CS1, mass spectrometry and SDS-PAGE | Streptomyces clavuligerus |
| 1.14.11.21 | monomer | 1 * 35839-38000, CS3, mass spectrometry and SDS-PAGE | Streptomyces antibioticus |
| 1.14.11.21 | monomer | 1 * 35750-44000, CS2, mass spectrometry and SDS-PAGE | Streptomyces clavuligerus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.21 | CS | - |
Streptomyces antibioticus |
| 1.14.11.21 | CS | - |
Streptomyces clavuligerus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.21 | 7 | - |
assay at | Streptomyces antibioticus |
| 1.14.11.21 | 7 | - |
assay at | Streptomyces clavuligerus |
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