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Literature summary extracted from

  • Barnsley, E.A.
    Phthalate pathway of phenanthrene metabolism: formation of 2-carboxybenzalpyruvate (1983), J. Bacteriol., 154, 113-117.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.13.11.38 no stabilization by ethanol, acetone or glycerol up to 10% v/v Gram-negative coccus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.38 Fe3+
-
 Gram-negative coccus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.34 0.047
-
 2'-carboxybenzalpyruvate pH 7.0, 25°C uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.38 Fe2+ activation Gram-negative coccus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.34 71000
-
 gel filtration uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate uncultured bacterium i.e. 2'-carboxybenzalpyruvate 2-carboxybenzaldehyde + pyruvate
-
 ?
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate uncultured bacterium the enzyme is involved in the degradation of phenanthrene 2-carboxybenzaldehyde + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.38 Gram-negative coccus
-
 isolated from soil and able to grow on phenanthrene as sole source of carbon and energy
-
1.13.11.38 Gram-negative coccus B156
-
 isolated from soil and able to grow on phenanthrene as sole source of carbon and energy
-
4.1.2.34 uncultured bacterium
-
 gram-negative coccus, strain B156
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.38 strain B156, isolated from soil and able to grow on phenanthrene as sole source of carbon and energy, partial purification Gram-negative coccus
4.1.2.34 partial uncultured bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.34 0.001
-
 succinate as carbon source uncultured bacterium
4.1.2.34 0.029
-
 protocatechuate or phenanthrene as carbon source uncultured bacterium
4.1.2.34 0.12
-
 phthalate as carbon source uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.38 1-hydroxy-2-naphthoate + O2
-
 Gram-negative coccus (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
-
 ?
1.13.11.38 1-hydroxy-2-naphthoate + O2
-
 Gram-negative coccus B156 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
-
 ?
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate i.e. 2'-carboxybenzalpyruvate uncultured bacterium 2-carboxybenzaldehyde + pyruvate
-
 ?
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate the enzyme is involved in the degradation of phenanthrene uncultured bacterium 2-carboxybenzaldehyde + pyruvate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.34 25
-
 assay at uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.38
-
-
 24 h, 90% loss of activity Gram-negative coccus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.34 7.3
-
 assay at uncultured bacterium