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Literature summary extracted from

  • Iwabuchi, T.; Harayama, S.
    Biochemical and genetic characterization of 2-carboxybenzaldehyde dehydrogenase, an enzyme involved in phenanthrene degradation by Nocardioides sp. strain KP7 (1997), J. Bacteriol., 179, 6488-6494.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.78 expression in Escherichia coli Nocardioides sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.78 Ca2+ 10 mM, about 30% inhibition Nocardioides sp.
1.2.1.78 EDTA 10 mM, 28% inhibition Nocardioides sp.
1.2.1.78 ethylenedioxybis(ethylamine)-N,N,N',N'-tetraacetic acid 10 mM, 14% inhibition Nocardioides sp.
1.2.1.78 Mg2+ 10 mM, about 30% inhibition Nocardioides sp.
1.2.1.78 Mn2+ 10 mM, about 30% inhibition Nocardioides sp.
1.2.1.78 additional information less than 15% inhibition in presence of 10 mM Zn2+, Co2+ or Cu2+ Nocardioides sp.
1.13.11.38 1,10-phenanthroline
-
 Nocardioides sp.
1.13.11.38 3-hydroxy-2-naphthoate
-
 Nocardioides sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.78 0.083
-
 NAD+ pH 9.4, 25°C Nocardioides sp.
1.2.1.78 0.1
-
 2-carboxybenzaldehyde pH 9.4, 25°C Nocardioides sp.
1.2.1.78 2.8
-
 2-carboxybenzaldehyde pH 9.4, 25°C Nocardioides sp.
1.13.11.38 0.01
-
 1-hydroxy-2-naphthoate
-
 Nocardioides sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.38 Iron contains 1.15 mol of iron per mol of enzyme Nocardioides sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.78 53000
-
 4 * 53000, SDS-PAGE Nocardioides sp.
1.2.1.78 205000
-
 gel filtration Nocardioides sp.
1.13.11.38 45000
-
 6 * 45000, SDS-PAGE Nocardioides sp.
1.13.11.38 270000
-
 gel filtration Nocardioides sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O Nocardioides sp. the enzyme is involved in phenanthrene degradation o-phthalic acid + NADH + H+
-
 ?
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O Nocardioides sp. KP7 the enzyme is involved in phenanthrene degradation o-phthalic acid + NADH + H+
-
 ?
1.13.11.38 1-hydroxy-2-naphthoate + O2 Nocardioides sp. enzyme is involved in degradation of phenanthrene (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
-
 ?
1.13.11.38 1-hydroxy-2-naphthoate + O2 Nocardioides sp. KP7 enzyme is involved in degradation of phenanthrene (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.78 Nocardioides sp. Q79EM7
-
-
1.2.1.78 Nocardioides sp. KP7 Q79EM7
-
-
1.13.11.38 Nocardioides sp.
-
-
-
1.13.11.38 Nocardioides sp. KP7
-
-
-
4.1.2.34 Nocardioides sp. Q79EM8
-
-
4.1.2.34 Nocardioides sp. KP7 Q79EM8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.78
-
 Nocardioides sp.
1.13.11.38
-
 Nocardioides sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.78 culture condition:phenanthrene-grown cell
-
 Nocardioides sp.
-
4.1.2.34 culture condition:phenanthrene-grown cell
-
 Nocardioides sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.38 180
-
-
 Nocardioides sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O
-
 Nocardioides sp. o-phthalic acid + NADH + H+
-
 ?
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O the enzyme is involved in phenanthrene degradation Nocardioides sp. o-phthalic acid + NADH + H+
-
 ?
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O
-
 Nocardioides sp. KP7 o-phthalic acid + NADH + H+
-
 ?
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O the enzyme is involved in phenanthrene degradation Nocardioides sp. KP7 o-phthalic acid + NADH + H+
-
 ?
1.2.1.78 2-nitrobenzaldehyde + NAD+ + H2O
-
 Nocardioides sp. ?
-
 ?
1.2.1.78 2-nitrobenzaldehyde + NAD+ + H2O
-
 Nocardioides sp. KP7 ?
-
 ?
1.2.1.78 additional information no activity with: 3-carboxybenzaldehyde, 4-carboxybenzaldehyde, benzaldehyde, salicylaldehyde, 2-methylbenzaldehyde, 2-chlorobenzaldehyde, 2-fluorobenzaldehyde, 2-benzaldehydesulfonate, 2-methoxybenzaldehyde, 1-hydroxy-2-naphthoaldehyde and n-hexylaldehyde Nocardioides sp. ?
-
 ?
1.2.1.78 additional information no activity with: 3-carboxybenzaldehyde, 4-carboxybenzaldehyde, benzaldehyde, salicylaldehyde, 2-methylbenzaldehyde, 2-chlorobenzaldehyde, 2-fluorobenzaldehyde, 2-benzaldehydesulfonate, 2-methoxybenzaldehyde, 1-hydroxy-2-naphthoaldehyde and n-hexylaldehyde Nocardioides sp. KP7 ?
-
 ?
1.13.11.38 1-hydroxy-2-naphthoate + O2
-
 Nocardioides sp. (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
-
 ?
1.13.11.38 1-hydroxy-2-naphthoate + O2 enzyme is involved in degradation of phenanthrene Nocardioides sp. (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
-
 ?
1.13.11.38 1-hydroxy-2-naphthoate + O2
-
 Nocardioides sp. KP7 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
-
 ?
1.13.11.38 1-hydroxy-2-naphthoate + O2 enzyme is involved in degradation of phenanthrene Nocardioides sp. KP7 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
-
 ?
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O i.e. 2'-carboxybenzalpyruvate Nocardioides sp. 2-carboxybenzaldehyde + pyruvate i.e.2-carboxybenzaldehyde + pyruvate r
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O i.e. 2'-carboxybenzalpyruvate Nocardioides sp. KP7 2-carboxybenzaldehyde + pyruvate i.e.2-carboxybenzaldehyde + pyruvate r

Subunits

EC Number Subunits Comment Organism
1.2.1.78 tetramer 4 * 53000, SDS-PAGE Nocardioides sp.
1.13.11.38 hexamer 6 * 45000, SDS-PAGE Nocardioides sp.

Synonyms

EC Number Synonyms Comment Organism
1.2.1.78 phdK
-
 Nocardioides sp.
4.1.2.34 phdJ
-
 Nocardioides sp.
4.1.2.34 trans-2'-carboxybenzalpyruvate aldolase
-
 Nocardioides sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.78 40
-
-
 Nocardioides sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.78 32
-
 NAD+ pH 9.4, 25°C Nocardioides sp.
1.2.1.78 39
-
 2-carboxybenzaldehyde pH 9.4, 25°C Nocardioides sp.
1.2.1.78 48
-
 2-nitrobenzaldehyde pH 9.4, 25°C Nocardioides sp.
1.13.11.38 114
-
 1-hydroxy-2-naphthoate
-
 Nocardioides sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.78 9
-
-
 Nocardioides sp.
1.13.11.38 7.5
-
-
 Nocardioides sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.78 NAD+
-
 Nocardioides sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.38 0.022
-
 3-hydroxy-2-naphthoate
-
 Nocardioides sp.