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Literature summary extracted from

  • Gorlatova, N.; Tchorzewski, M.; Kurihara, T.; Soda, K.; Esaki, N.
    Purification, characterization, and mechanism of a flavin mononucleotide-dependent 2-nitropropane dioxygenase from Neurospora crassa (1998), Appl. Environ. Microbiol., 64, 1029-1033.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.16 2-mercaptoethanol 1 mM, relative activity remaining 2% Neurospora crassa
1.13.12.16 cytochrome c 0.04 mM, relative activity remaining 34% Neurospora crassa
1.13.12.16 dithiothreitol 1 mM, complete inhibition Neurospora crassa
1.13.12.16 EDTA 1 mM, relative activity remaining 95% Neurospora crassa
1.13.12.16 GSH 5 mM, relative activity remaining 28% Neurospora crassa
1.13.12.16 iodoacetate 1 mM, complete inhibition Neurospora crassa
1.13.12.16 Nitro blue tetrazolium 2.5 mM, relative activity remaining 10% Neurospora crassa
1.13.12.16 Superoxide dismutase complete inhibition Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.16 2 3 nitroethane neutral form of substrate Neurospora crassa
1.13.12.16 3.1
-
 2-Nitropropane anionic form of substrate Neurospora crassa
1.13.12.16 3.1
-
 propyl-2-nitronate anionic form of substrate Neurospora crassa
1.13.12.16 6
-
 2-Nitropropane anionic form of substrate Neurospora crassa
1.13.12.16 6
-
 ethyl nitronate anionic form of substrate Neurospora crassa
1.13.12.16 8.3
-
 1-Nitropropane anionic form of substrate Neurospora crassa
1.13.12.16 8.3
-
 propyl-1-nitronate anionic form of substrate Neurospora crassa
1.13.12.16 20
-
 1-Nitropropane neutral form of substrate Neurospora crassa
1.13.12.16 23
-
 nitroethane neutral form of substrate Neurospora crassa
1.13.12.16 33
-
 2-Nitropropane neutral form of substrate Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.16 40000
-
 2 * 40000, SDS-PAGE Neurospora crassa
1.13.12.16 70000
-
 gel filtration Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.16 Neurospora crassa
-
-
-
1.13.12.16 Neurospora crassa
-
 ATCC 10337
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.16
-
 Neurospora crassa
1.13.12.16 to homogeneity Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.12.16 mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.16 10000
-
-
 Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.16 1-hydroxybutyl-2-nitronate + O2
-
 Neurospora crassa 3-hydroxy-butane-2-one + HNO2
-
 ?
1.13.12.16 1-hydroxyethyl-2-nitronate + O2
-
 Neurospora crassa glycoaldehyde + HNO2
-
 ?
1.13.12.16 1-nitropentane + O2 3% of the activity with 2-nitropropane Neurospora crassa ? + HNO2
-
 ?
1.13.12.16 1-nitropropane + O2 21% of the activity with 2-nitropropane Neurospora crassa propionaldehyde + HNO2
-
 ?
1.13.12.16 2-hydroxypentyl-3-nitronate + O2
-
 Neurospora crassa 2-hydroxy-pentane-3-one + HNO2
-
 ?
1.13.12.16 2-nitroethanol + O2 13% of the activity with 2-nitropropane Neurospora crassa glycoaldehyde + HNO2
-
 ?
1.13.12.16 2-nitropropane + O2
-
 Neurospora crassa acetone + HNO2
-
 ?
1.13.12.16 3-nitro-2-butanol + O2 14% of the activity with 2-nitropropane Neurospora crassa 3-hydroxy-butane-2-one + HNO2
-
 ?
1.13.12.16 3-nitro-2-pentanol + O2 20% of the activity with 2-nitropropane Neurospora crassa 2-hydroxy-pentane-3-one + HNO2
-
 ?
1.13.12.16 3-nitropropionic acid + O2 12% of the activity with 2-nitropropane Neurospora crassa ?
-
 ?
1.13.12.16 cyclohexyl nitronate + O2
-
 Neurospora crassa ? + HNO2
-
 ?
1.13.12.16 ethyl nitronate + O2
-
 Neurospora crassa acetaldehyde + HNO2
-
 ?
1.13.12.16 additional information anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds Neurospora crassa ?
-
 ?
1.13.12.16 additional information anionic forms of nitroalkanes are much better substrates than are neutral forms, enzyme does not act on aromatic compounds. Measuring nitrite production with 20 mM anionic nitro compounds as substrates Neurospora crassa ?
-
 ?
1.13.12.16 nitrocyclohexane + O2 2% of the activity with 2-nitropropane Neurospora crassa ? + HNO2
-
 ?
1.13.12.16 nitroethane + O2 27% of the activity with 2-nitropropane Neurospora crassa acetaldehyde + HNO2
-
 ?
1.13.12.16 pentyl-1-nitronate + O2
-
 Neurospora crassa ? + HNO2
-
 ?
1.13.12.16 propionate-3-nitronate + O2
-
 Neurospora crassa ?
-
 ?
1.13.12.16 propyl-1-nitronate + O2
-
 Neurospora crassa propionaldehyde + HNO2
-
 ?
1.13.12.16 propyl-2-nitronate + O2
-
 Neurospora crassa acetone + HNO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.16 dimer 2 * 40000, SDS-PAGE Neurospora crassa

Synonyms

EC Number Synonyms Comment Organism
1.13.12.16 2-nitropropane dioxygenase
-
 Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.16 30
-
 assay at Neurospora crassa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.12.16 32
-
 nitroethane neutral form of substrate Neurospora crassa
1.13.12.16 35
-
 1-Nitropropane neutral form of substrate Neurospora crassa
1.13.12.16 160
-
 2-Nitropropane neutral form of substrate Neurospora crassa
1.13.12.16 1300
-
 1-Nitropropane anionic form of substrate Neurospora crassa
1.13.12.16 1300
-
 propyl-1-nitronate anionic form of substrate Neurospora crassa
1.13.12.16 2000
-
 nitroethane anionic form of substrate Neurospora crassa
1.13.12.16 2000
-
 ethyl nitronate anionic form of substrate Neurospora crassa
1.13.12.16 6400
-
 2-Nitropropane anionic form of substrate Neurospora crassa
1.13.12.16 6400
-
 propyl-2-nitronate anionic form of substrate Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.16 6.5
-
 assay at Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.16 FMN 2 mol of FMN per mol of subunit Neurospora crassa
1.13.12.16 FMN dependent, enzyme contains flavin mononucleotide as a prosthetic group, 2 mol of FMN per mol of subunit Neurospora crassa