EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.12.16 | 0.3 | - |
O2 | - |
Cyberlindnera mrakii | |
1.13.12.16 | 0.59 | - |
3-Nitro-2-butanol | anionic form of substrate | Cyberlindnera mrakii | |
1.13.12.16 | 0.59 | - |
3-Nitro-2-butanol | anionic form of the substrate | Cyberlindnera mrakii | |
1.13.12.16 | 1.04 | - |
3-Nitro-2-pentanol | anionic form of substrate | Cyberlindnera mrakii | |
1.13.12.16 | 1.04 | - |
3-Nitro-2-pentanol | anionic form of the substrate | Cyberlindnera mrakii | |
1.13.12.16 | 1.61 | - |
2-Nitropropane | anionic form of substrate | Cyberlindnera mrakii | |
1.13.12.16 | 1.61 | - |
2-Nitropropane | anionic form of the substrate | Cyberlindnera mrakii | |
1.13.12.16 | 3.13 | - |
nitroethane | anionic form of substrate | Cyberlindnera mrakii | |
1.13.12.16 | 3.13 | - |
nitroethane | anionic form of the substrate | Cyberlindnera mrakii | |
1.13.12.16 | 3.23 | - |
1-Nitropropane | anionic form of substrate | Cyberlindnera mrakii | |
1.13.12.16 | 3.23 | - |
1-Nitropropane | anionic form of the substrate | Cyberlindnera mrakii | |
1.13.12.16 | 4.2 | - |
3-Nitro-2-butanol | - |
Cyberlindnera mrakii | |
1.13.12.16 | 4.2 | - |
3-Nitro-2-butanol | neutral form of the substrate | Cyberlindnera mrakii | |
1.13.12.16 | 6.8 | - |
nitroethane | neutral form of substrate | Cyberlindnera mrakii | |
1.13.12.16 | 6.8 | - |
nitroethane | neutral form of the substrate | Cyberlindnera mrakii | |
1.13.12.16 | 21.3 | - |
2-Nitropropane | neutral form of substrate | Cyberlindnera mrakii | |
1.13.12.16 | 21.3 | - |
2-Nitropropane | neutral form of the substrate | Cyberlindnera mrakii | |
1.13.12.16 | 24.3 | - |
nitroethane | neutral form of substrate | Cyberlindnera mrakii | |
1.13.12.16 | 24.3 | - |
nitroethane | neutral form of the substrate | Cyberlindnera mrakii | |
1.13.12.16 | 25.6 | - |
1-Nitropropane | neutral form of substrate | Cyberlindnera mrakii | |
1.13.12.16 | 25.6 | - |
1-Nitropropane | neutral form of the substrate | Cyberlindnera mrakii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.12.16 | Fe | enzyme contains 1.09 gatom of iron per mol of enzyme | Cyberlindnera mrakii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.12.16 | 42000 | - |
sedimentation equilibrium centrifugation | Cyberlindnera mrakii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.16 | additional information | Cyberlindnera mrakii | enzyme catalyzes the oxygenative denitrification of anionic nitroalkanes much more effectively than that of the neutral ones | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.12.16 | Cyberlindnera mrakii | - |
- |
- |
1.13.12.16 | Cyberlindnera mrakii | Q12723 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.12.16 | - |
Cyberlindnera mrakii |
1.13.12.16 | to homogeneity | Cyberlindnera mrakii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.12.16 | 397 | - |
- |
Cyberlindnera mrakii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.16 | 1-nitropropane + O2 | 57.9% of activity with 2-nitropropane | Cyberlindnera mrakii | propionaldehyde + HNO2 | - |
? | |
1.13.12.16 | 1-nitropropane + O2 | 57.9% of activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | propionaldehyde + HNO2 | - |
? | |
1.13.12.16 | 2-nitro-1-butanol + O2 | 31.5% of the activity with 2-nitropropane | Cyberlindnera mrakii | 1-hydroxy-butane-2-one + HNO2 | - |
? | |
1.13.12.16 | 2-nitro-1-butanol + O2 | 2.7% of the activity with 2-nitropropane | Cyberlindnera mrakii | 1-hydroxy-butane-2-one + HNO2 | - |
? | |
1.13.12.16 | 2-nitro-1-butanol + O2 | 2.7% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | 1-hydroxy-butane-2-one + HNO2 | - |
? | |
1.13.12.16 | 2-nitro-1-butanol + O2 | 31.5% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | 1-hydroxy-butane-2-one + HNO2 | - |
? | |
1.13.12.16 | 2-nitro-1-propanol + O2 | 7.7% of activity with 2-nitropropane | Cyberlindnera mrakii | 1-hydroxy-propane-2-one + HNO2 | - |
? | |
1.13.12.16 | 2-nitro-1-propanol + O2 | 7.7% of activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | 1-hydroxy-propane-2-one + HNO2 | - |
? | |
1.13.12.16 | 2-nitropropane + O2 | - |
Cyberlindnera mrakii | acetone + HNO2 | - |
? | |
1.13.12.16 | 3-nitro-2-butanol + O2 | 26.7% of the activity with 2-nitropropane | Cyberlindnera mrakii | 3-hydroxy-butane-2-one + HNO2 | - |
? | |
1.13.12.16 | 3-nitro-2-butanol + O2 | 26.7% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | 3-hydroxy-butane-2-one + HNO2 | - |
? | |
1.13.12.16 | 3-nitro-2-pentanol + O2 | 32.3% of the activity with 2-nitropropane | Cyberlindnera mrakii | 2-hydroxy-pentane-3-one + HNO2 | - |
? | |
1.13.12.16 | 3-nitro-2-pentanol + O2 | 32.3% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | 2-hydroxy-pentane-3-one + HNO2 | - |
? | |
1.13.12.16 | 3-nitropropionic acid + O2 | 25.5% of the activity with 2-nitropropane | Cyberlindnera mrakii | ? | - |
? | |
1.13.12.16 | 3-nitropropionic acid + O2 | 25.5% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | ? | - |
? | |
1.13.12.16 | additional information | enzyme catalyzes the oxygenative denitrification of anionic nitroalkanes much more effectively than that of the neutral ones | Cyberlindnera mrakii | ? | - |
? | |
1.13.12.16 | nitrocyclohexane + O2 | 1.5% of the activity with 2-nitropropane | Cyberlindnera mrakii | cyclohexanone + HNO2 | - |
? | |
1.13.12.16 | nitrocyclohexane + O2 | 1.5% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | cyclohexanone + HNO2 | - |
? | |
1.13.12.16 | nitroethane + O2 | 4.2% of the activity with 2-nitropropane | Cyberlindnera mrakii | acetaldehyde + HNO2 | - |
? | |
1.13.12.16 | nitroethane + O2 | 4.2% of the activity with 2-nitropropane (anionic form) | Cyberlindnera mrakii | acetaldehyde + HNO2 | - |
? | |
1.13.12.16 | nitromethane + O2 | 4.2% of the activity with 2-nitropropane activity | Cyberlindnera mrakii | formaldehyde + HNO2 | - |
? | |
1.13.12.16 | nitromethane + O2 | 4.2% of the activity with 2-nitropropane activity (anionic form) | Cyberlindnera mrakii | formaldehyde + HNO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.12.16 | monomer | 1 * 42000, SDS-PAGE | Cyberlindnera mrakii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.12.16 | 2-nitropropane dioxygenase | - |
Cyberlindnera mrakii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.12.16 | 6.5 | - |
oxidation of anionic form of 2-nitropropane | Cyberlindnera mrakii |
1.13.12.16 | 8 | - |
oxidation of neutral form of 2-nitropropane | Cyberlindnera mrakii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.13.12.16 | FAD | contains 1 mol FAD per mol of enzynme | Cyberlindnera mrakii | |
1.13.12.16 | FAD | contains 1 mol FAD per mol of enzyme | Cyberlindnera mrakii |