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Literature summary extracted from

  • Kido, T.; Tanizawa, K.; Inagaki, K.; Yoshimura, T.; Ishida, M.; Hashizume, K.; Soda, K.
    2-Nitropropane dioxygenase from Hansenula mrakii: re-characterization of the enzyme and oxidation of anionic nitroalkanes (1984), Agric. Biol. Chem., 48, 2549-2554.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.16 0.3
-
O2
-
Cyberlindnera mrakii
1.13.12.16 0.59
-
3-Nitro-2-butanol anionic form of substrate Cyberlindnera mrakii
1.13.12.16 0.59
-
3-Nitro-2-butanol anionic form of the substrate Cyberlindnera mrakii
1.13.12.16 1.04
-
3-Nitro-2-pentanol anionic form of substrate Cyberlindnera mrakii
1.13.12.16 1.04
-
3-Nitro-2-pentanol anionic form of the substrate Cyberlindnera mrakii
1.13.12.16 1.61
-
2-Nitropropane anionic form of substrate Cyberlindnera mrakii
1.13.12.16 1.61
-
2-Nitropropane anionic form of the substrate Cyberlindnera mrakii
1.13.12.16 3.13
-
nitroethane anionic form of substrate Cyberlindnera mrakii
1.13.12.16 3.13
-
nitroethane anionic form of the substrate Cyberlindnera mrakii
1.13.12.16 3.23
-
1-Nitropropane anionic form of substrate Cyberlindnera mrakii
1.13.12.16 3.23
-
1-Nitropropane anionic form of the substrate Cyberlindnera mrakii
1.13.12.16 4.2
-
3-Nitro-2-butanol
-
Cyberlindnera mrakii
1.13.12.16 4.2
-
3-Nitro-2-butanol neutral form of the substrate Cyberlindnera mrakii
1.13.12.16 6.8
-
nitroethane neutral form of substrate Cyberlindnera mrakii
1.13.12.16 6.8
-
nitroethane neutral form of the substrate Cyberlindnera mrakii
1.13.12.16 21.3
-
2-Nitropropane neutral form of substrate Cyberlindnera mrakii
1.13.12.16 21.3
-
2-Nitropropane neutral form of the substrate Cyberlindnera mrakii
1.13.12.16 24.3
-
nitroethane neutral form of substrate Cyberlindnera mrakii
1.13.12.16 24.3
-
nitroethane neutral form of the substrate Cyberlindnera mrakii
1.13.12.16 25.6
-
1-Nitropropane neutral form of substrate Cyberlindnera mrakii
1.13.12.16 25.6
-
1-Nitropropane neutral form of the substrate Cyberlindnera mrakii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.16 Fe enzyme contains 1.09 gatom of iron per mol of enzyme Cyberlindnera mrakii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.16 42000
-
sedimentation equilibrium centrifugation Cyberlindnera mrakii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.16 additional information Cyberlindnera mrakii enzyme catalyzes the oxygenative denitrification of anionic nitroalkanes much more effectively than that of the neutral ones ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.16 Cyberlindnera mrakii
-
-
-
1.13.12.16 Cyberlindnera mrakii Q12723
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.16
-
Cyberlindnera mrakii
1.13.12.16 to homogeneity Cyberlindnera mrakii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.16 397
-
-
Cyberlindnera mrakii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.16 1-nitropropane + O2 57.9% of activity with 2-nitropropane Cyberlindnera mrakii propionaldehyde + HNO2
-
?
1.13.12.16 1-nitropropane + O2 57.9% of activity with 2-nitropropane (anionic form) Cyberlindnera mrakii propionaldehyde + HNO2
-
?
1.13.12.16 2-nitro-1-butanol + O2 31.5% of the activity with 2-nitropropane Cyberlindnera mrakii 1-hydroxy-butane-2-one + HNO2
-
?
1.13.12.16 2-nitro-1-butanol + O2 2.7% of the activity with 2-nitropropane Cyberlindnera mrakii 1-hydroxy-butane-2-one + HNO2
-
?
1.13.12.16 2-nitro-1-butanol + O2 2.7% of the activity with 2-nitropropane (anionic form) Cyberlindnera mrakii 1-hydroxy-butane-2-one + HNO2
-
?
1.13.12.16 2-nitro-1-butanol + O2 31.5% of the activity with 2-nitropropane (anionic form) Cyberlindnera mrakii 1-hydroxy-butane-2-one + HNO2
-
?
1.13.12.16 2-nitro-1-propanol + O2 7.7% of activity with 2-nitropropane Cyberlindnera mrakii 1-hydroxy-propane-2-one + HNO2
-
?
1.13.12.16 2-nitro-1-propanol + O2 7.7% of activity with 2-nitropropane (anionic form) Cyberlindnera mrakii 1-hydroxy-propane-2-one + HNO2
-
?
1.13.12.16 2-nitropropane + O2
-
Cyberlindnera mrakii acetone + HNO2
-
?
1.13.12.16 3-nitro-2-butanol + O2 26.7% of the activity with 2-nitropropane Cyberlindnera mrakii 3-hydroxy-butane-2-one + HNO2
-
?
1.13.12.16 3-nitro-2-butanol + O2 26.7% of the activity with 2-nitropropane (anionic form) Cyberlindnera mrakii 3-hydroxy-butane-2-one + HNO2
-
?
1.13.12.16 3-nitro-2-pentanol + O2 32.3% of the activity with 2-nitropropane Cyberlindnera mrakii 2-hydroxy-pentane-3-one + HNO2
-
?
1.13.12.16 3-nitro-2-pentanol + O2 32.3% of the activity with 2-nitropropane (anionic form) Cyberlindnera mrakii 2-hydroxy-pentane-3-one + HNO2
-
?
1.13.12.16 3-nitropropionic acid + O2 25.5% of the activity with 2-nitropropane Cyberlindnera mrakii ?
-
?
1.13.12.16 3-nitropropionic acid + O2 25.5% of the activity with 2-nitropropane (anionic form) Cyberlindnera mrakii ?
-
?
1.13.12.16 additional information enzyme catalyzes the oxygenative denitrification of anionic nitroalkanes much more effectively than that of the neutral ones Cyberlindnera mrakii ?
-
?
1.13.12.16 nitrocyclohexane + O2 1.5% of the activity with 2-nitropropane Cyberlindnera mrakii cyclohexanone + HNO2
-
?
1.13.12.16 nitrocyclohexane + O2 1.5% of the activity with 2-nitropropane (anionic form) Cyberlindnera mrakii cyclohexanone + HNO2
-
?
1.13.12.16 nitroethane + O2 4.2% of the activity with 2-nitropropane Cyberlindnera mrakii acetaldehyde + HNO2
-
?
1.13.12.16 nitroethane + O2 4.2% of the activity with 2-nitropropane (anionic form) Cyberlindnera mrakii acetaldehyde + HNO2
-
?
1.13.12.16 nitromethane + O2 4.2% of the activity with 2-nitropropane activity Cyberlindnera mrakii formaldehyde + HNO2
-
?
1.13.12.16 nitromethane + O2 4.2% of the activity with 2-nitropropane activity (anionic form) Cyberlindnera mrakii formaldehyde + HNO2
-
?

Subunits

EC Number Subunits Comment Organism
1.13.12.16 monomer 1 * 42000, SDS-PAGE Cyberlindnera mrakii

Synonyms

EC Number Synonyms Comment Organism
1.13.12.16 2-nitropropane dioxygenase
-
Cyberlindnera mrakii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.16 6.5
-
oxidation of anionic form of 2-nitropropane Cyberlindnera mrakii
1.13.12.16 8
-
oxidation of neutral form of 2-nitropropane Cyberlindnera mrakii

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.16 FAD contains 1 mol FAD per mol of enzynme Cyberlindnera mrakii
1.13.12.16 FAD contains 1 mol FAD per mol of enzyme Cyberlindnera mrakii