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Literature summary extracted from

  • Igarashi, S.; Ohtera, T.; Yoshida, H.; Witarto, A.B.; Sode, K.
    Construction and characterization of mutant water-soluble PQQ glucose dehydrogenases with altered K(m) values--site-directed mutagenesis studies on the putative active site (1999), Biochem. Biophys. Res. Commun., 264, 820-824.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.2 D276E drastic decrease in EDTA tolerance Acinetobacter calcoaceticus
1.1.5.2 E277A decreased Km value for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 E277D decreased Km value for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 E277G drastic decrease in EDTA tolerance Acinetobacter calcoaceticus
1.1.5.2 E277H decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 E277K decreased Km value for glucose and altered substrate specificity, significantly increased catalytic efficiency compared with the wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 E277N decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 E277Q decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 E277V decreased Km values for glucose and altered substrate specificity, thermal stability is less than 20% of that of the wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 N275E drastic decrease in EDTA tolerance Acinetobacter calcoaceticus
1.1.99.35 D275E substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 6%, D-mannose 11%, D-allose 44%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 7%, D-lactose 52%, D-maltose 44%, respectively Acinetobacter calcoaceticus
1.1.99.35 D276E substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 13%, D-mannose 19%, D-allose 54%, 3-O-methyl-D-glucose 57%, D-galactose 26%, D-xylose 20%, D-lactose 75%, D-maltose 66%, respectively Acinetobacter calcoaceticus
1.1.99.35 E277A substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 11%, D-mannose 33%, D-allose 142%, 3-O-methyl-D-glucose 91%, D-galactose 46%, D-xylose 23%, D-lactose 83%, D-maltose 39%, respectively Acinetobacter calcoaceticus
1.1.99.35 E277D substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 9%, D-mannose 19%, D-allose 68%, 3-O-methyl-D-glucose 58%, D-galactose 21%, D-xylose 15%, D-lactose 87%, D-maltose 73%, respectively Acinetobacter calcoaceticus
1.1.99.35 E277G substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 15%, D-mannose 38%, D-allose 189%, 3-O-methyl-D-glucose 113%, D-galactose 59%, D-xylose 28%, D-lactose 100%, D-maltose 52%, respectively Acinetobacter calcoaceticus
1.1.99.35 E277H substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 3%, D-mannose 11%, D-allose 67%, 3-O-methyl-D-glucose 45%, D-galactose 12%, D-xylose 9%, D-lactose 57%, D-maltose 39%, respectively Acinetobacter calcoaceticus
1.1.99.35 E277K substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 7%, D-mannose 18%, D-allose 116%, 3-O-methyl-D-glucose 84%, D-galactose 28%, D-xylose 18%, D-lactose 79%, D-maltose 74%, respectively Acinetobacter calcoaceticus
1.1.99.35 E277N substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 148%, 3-O-methyl-D-glucose 91%, D-galactose 43%, D-xylose 18%, D-lactose 86%, D-maltose 59%, respectively Acinetobacter calcoaceticus
1.1.99.35 E277Q substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 10%, D-mannose 31%, D-allose 134%, 3-O-methyl-D-glucose 80%, D-galactose 35%, D-xylose 25%, D-lactose 68%, D-maltose 54%, respectively Acinetobacter calcoaceticus
1.1.99.35 E277V substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 8%, D-mannose 29%, D-allose 150%, 3-O-methyl-D-glucose 101%, D-galactose 25%, D-xylose 19%, D-lactose 114%, D-maltose 65%, respectively Acinetobacter calcoaceticus
1.1.99.35 I278F substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 4%, D-mannose 14%, D-allose 54%, 3-O-methyl-D-glucose 47%, D-galactose 15%, D-xylose 12%, D-lactose 64%, D-maltose 49%, respectively Acinetobacter calcoaceticus
1.1.99.35 N279H substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 6%, D-allose 49%, 3-O-methyl-D-glucose 50%, D-galactose 13%, D-xylose 8%, D-lactose 64%, D-maltose 61%, respectively Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 0.3
-
 D-glucose pH 7.0, mutant enzyme E277G Acinetobacter calcoaceticus
1.1.5.2 1.2
-
 D-glucose mutant E277N Acinetobacter calcoaceticus
1.1.5.2 1.5
-
 D-glucose mutant enzyme E277A Acinetobacter calcoaceticus
1.1.5.2 2.5
-
 D-glucose pH 7.0, mutant enzyme E277V Acinetobacter calcoaceticus
1.1.5.2 4.3
-
 D-glucose pH 7.0, mutant enzyme E277Q Acinetobacter calcoaceticus
1.1.5.2 6.8
-
 D-galactose pH 7.0, wild-type enzyme and mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 7
-
 D-glucose pH 7.0, mutant enzyme I278F Acinetobacter calcoaceticus
1.1.5.2 7.4
-
 D-glucose pH 7.0, mutant enzyme E277D Acinetobacter calcoaceticus
1.1.5.2 7.5
-
 lactose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 7.7
-
 D-glucose pH 7.0, mutant enzyme E277H Acinetobacter calcoaceticus
1.1.5.2 8.8
-
 D-glucose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 8.9
-
 D-glucose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 14.3
-
 maltose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 14.3
-
 lactose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 14.3
-
 D-xylose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 15.7
-
 D-glucose pH 7.0, mutant enzyme N279H Acinetobacter calcoaceticus
1.1.5.2 21
-
 allose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 22
-
 D-mannose pH 7.0, wild-type enzyme and mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 24
-
 D-glucose pH 7.0, mutant enzyme D275E and D276E Acinetobacter calcoaceticus
1.1.5.2 26
-
 D-glucose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 27
-
 3-O-methyl-D-glucose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 29
-
 D-allose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 30.9
-
 maltose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 34
-
 D-xylose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 46
-
 3-O-methyl-D-glucose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 88
-
 2-deoxy-D-glucose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 90
-
 2-deoxy-D-glucose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.99.35 6.8
-
 D-galactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 6.8
-
 D-galactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 7.5
-
 D-xylose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 8.8
-
 D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 14.3
-
 D-xylose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 14.3
-
 D-lactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 14.3
-
 D-maltose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 21
-
 D-allose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 22
-
 D-mannose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 22
-
 D-mannose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 26.8
-
 D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 27
-
 3-O-methyl-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 29
-
 D-allose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 30.9
-
 D-maltose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 34
-
 D-lactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 46
-
 3-O-methyl-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 88
-
 2-deoxy-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 90
-
 2-deoxy-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Acinetobacter calcoaceticus
-
 expression in Escherichia coli
-
1.1.99.35 Acinetobacter calcoaceticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 2-deoxy-D-glucose + pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 3-O-methyl-D-glucose + pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 D-allose + pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus D-allono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 D-galactose + pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus D-galactono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 D-glucose + 2,6-dichlorophenol-indophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + phenazine methosulfate
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-mannose + pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus D-mannono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 D-xylose + pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus D-xylono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 lactose + pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.5.2 maltose + pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.99.35 2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol 4% of the activity with D-glucose Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol 81% of the activity with D-glucose Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-allose + oxidized 2,6-dichlorophenolindophenol 47% of the activity with D-glucose Acinetobacter calcoaceticus D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-galactose + oxidized 2,6-dichlorophenolindophenol 11% of the activity with D-glucose Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-glucose + oxidized 2,6-dichlorophenolindophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-mannose + oxidized 2,6-dichlorophenolindophenol 13% of the activity with D-glucose Acinetobacter calcoaceticus D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-xylose + oxidized 2,6-dichlorophenolindophenol 7% of the activity with D-glucose Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 lactose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 maltose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.5.2 55
-
 t1/2: 10 min for wild-type enzyme and mutant enzyme E277K, 4 min for mutant enzymes E277Q and N279H, 25 min for mutant enzyme I278F, less than 2 min for mutant enzymes E277G, E277A, E277D, E277H, E277N, E277V, D275E and D276E Acinetobacter calcoaceticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.2 121
-
 D-galactose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 201
-
 D-xylose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 267
-
 D-mannose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 331
-
 2-deoxy-D-glucose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 630
-
 D-galactose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 669
-
 lactose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 678
-
 D-xylose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 785
-
 maltose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 861
-
 D-mannose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 1020
-
 maltose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 1060
-
 2-deoxy-D-glucose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 1440
-
 D-allose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 1450
-
 3-O-methyl-D-glucose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 1800
-
 lactose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 3070
-
 D-glucose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 3200
-
 3-O-methyl-D-glucose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.5.2 3440
-
 D-glucose pH 7.0, wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 4560
-
 D-allose pH 7.0, mutant enzyme E277K Acinetobacter calcoaceticus
1.1.99.35 121
-
 D-galactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 201
-
 D-lactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 267
-
 D-mannose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 331
-
 2-deoxy-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 630
-
 D-galactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 669
-
 D-xylose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 678
-
 D-lactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 785
-
 D-maltose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 861
-
 D-mannose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1015
-
 D-maltose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1063
-
 2-deoxy-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1443
-
 D-allose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1448
-
 3-O-methyl-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 1795
-
 D-xylose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 3071
-
 D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 3198
-
 3-O-methyl-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 3436
-
 D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 4563
-
 D-allose mutant E277K, pH 7.0 Acinetobacter calcoaceticus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.35 4
-
 2-deoxy-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 7
-
 D-lactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 12
-
 2-deoxy-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 12
-
 D-mannose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 18
-
 D-galactose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 20
-
 D-lactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 25
-
 D-maltose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 31
-
 3-O-methyl-D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 39
-
 D-mannose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 47
-
 D-xylose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 50
-
 D-allose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 71
-
 D-maltose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 78
-
 D-galactose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 118
-
 3-O-methyl-D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 128
-
 D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 239
-
 D-xylose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 287
-
 D-allose mutant E277K, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 349
-
 D-glucose mutant E277K, pH 7.0 Acinetobacter calcoaceticus