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Literature summary extracted from

  • Biou, V.; Dumas, R.; Cohen-Addad, C.; Douce, R.; Job, D.; Pebay-Peyroula, E.
    The crystal structure of plant acetohydroxy acid isomeroreductase complexed with NADPH, two magnesium ions and a herbicidal transition state analog determined at 1.65 A resolution (1997), EMBO J., 16, 3405-3415.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.86 ammonium sulfate precipitation, crystal structure of the enzyme complexed with NADPH, two magnesium ions and N-hydroxy-N-isopropyloxamate, a herbicidal transition state analog determined at 1.65 A resolution, recombinant enzyme overexpressed in Escherichia coli Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.86 N-Hydroxy-N-isopropyloxamate competitive Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.86 Mg2+ required Spinacia oleracea
1.1.1.86 Mg2+ two ions bind to enzyme Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH Spinacia oleracea enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Spinacia oleracea
-
 overexpressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86 recombinant enzyme from Escherichia coli Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Spinacia oleracea 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r
1.1.1.86 NADPH + 2-acetolactate
-
 Spinacia oleracea NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADPH
-
 Spinacia oleracea