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Literature summary extracted from

  • Dumas, R.; Joyard, J.; Douce, R.
    Purification and characterization of acetohydroxyacid reductoisomerase from spinach chloroplasts (1989), Biochem. J., 262, 971-976.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.86 NADP+ competitive with NADPH Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.86 0.0057
-
 NADPH pH 8, 30°C, isoenzyme 1 Spinacia oleracea
1.1.1.86 0.025
-
 2-acetolactate pH 8, 30°C, isoenzyme 1 Spinacia oleracea
1.1.1.86 0.037
-
 2-aceto-2-hydroxybutyrate pH 8, 30°C, isoenzyme 1 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.86 chloroplast
-
 Spinacia oleracea 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.86 Mg2+ required Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.86 59000
-
 4 * 59000, SDS-PAGE Spinacia oleracea
1.1.1.86 205000
-
 SDS-PAGE, isoenzyme 3 Spinacia oleracea
1.1.1.86 220000
-
 SDS-PAGE, isoenzyme 2 Spinacia oleracea
1.1.1.86 235000
-
 SDS-PAGE, isoenzyme 1 Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH Spinacia oleracea enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Spinacia oleracea
-
 spinach, multiple enzyme forms: iso 1, 2 and 3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.86 3.79
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Spinacia oleracea 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r
1.1.1.86 NADPH + 2-aceto-2-hydroxybutyrate
-
 Spinacia oleracea NADP+ + ?
-
 ?
1.1.1.86 NADPH + 2-acetolactate
-
 Spinacia oleracea NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.86 tetramer 4 * 59000, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.86 7.5 8 reaction with 2-acetolactate, isoenzyme 1 Spinacia oleracea
1.1.1.86 8 8.5 reaction with 2-aceto-2-hydroxybutyrate Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.86 6.5 8.5 pH 6.5: about 35% of maximal activity, pH 8.5: about 90% of maximal activity, reaction with 2-acetolactate, isoenzyme 1 Spinacia oleracea
1.1.1.86 7 8.5 pH 7.0: about 25% of maximal activity, pH 8.0-8.5: optimum, isoenzyme 1 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADPH
-
 Spinacia oleracea