EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.86 | 2,3-dihydroxy-3-methylbutyrate | - |
Escherichia coli | |
1.1.1.86 | 2-Methyllactate | uncompetitive; uncompetitive with respect to NADPH | Escherichia coli | |
1.1.1.86 | 3-aminopyridine-NADP+ | - |
Escherichia coli | |
1.1.1.86 | Mn2+ | with acetolactate as substrate, Mn2+ behaves as a competitive inhibitor in presence of Mg2+ | Escherichia coli | |
1.1.1.86 | NADP+ | - |
Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.86 | Mg2+ | required | Escherichia coli | |
1.1.1.86 | Mg2+ | Km: 0.42 mM | Escherichia coli | |
1.1.1.86 | Mg2+ | with acetolactate as substrate , Mg2+ is the only divalent metal ion that supports enzyme catalysis | Escherichia coli | |
1.1.1.86 | Mn2+ | activates reaction with 3-hydroxy-3-methyl-2-oxobutanoate, no effect on reaction with acetolactate | Escherichia coli | |
1.1.1.86 | additional information | Mn2+, Co2+, Ni2+, Zn2+, Ca2+, Cu2+ and Co3+ can not substitute for Mg2+ | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.86 | 2-acetolactate + NADPH | Escherichia coli | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.86 | Escherichia coli | - |
recombinant | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.86 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.86 | 2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Escherichia coli | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
1.1.1.86 | NADPH + 2-acetolactate | - |
Escherichia coli | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.86 | NADPH | - |
Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.86 | additional information | - |
additional information | - |
Escherichia coli |