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Literature summary extracted from

  • Chunduru, S.K.; Mrachko, G.T.; Calvo, K.C.
    Mechanism of ketol acid reductoisomerase--steady-state analysis and metal ion requirement (1989), Biochemistry, 28, 486-493.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.86 2,3-dihydroxy-3-methylbutyrate
-
 Escherichia coli
1.1.1.86 2-Methyllactate uncompetitive; uncompetitive with respect to NADPH Escherichia coli
1.1.1.86 3-aminopyridine-NADP+
-
 Escherichia coli
1.1.1.86 Mn2+ with acetolactate as substrate, Mn2+ behaves as a competitive inhibitor in presence of Mg2+ Escherichia coli
1.1.1.86 NADP+
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.86 Mg2+ required Escherichia coli
1.1.1.86 Mg2+ Km: 0.42 mM Escherichia coli
1.1.1.86 Mg2+ with acetolactate as substrate , Mg2+ is the only divalent metal ion that supports enzyme catalysis Escherichia coli
1.1.1.86 Mn2+ activates reaction with 3-hydroxy-3-methyl-2-oxobutanoate, no effect on reaction with acetolactate Escherichia coli
1.1.1.86 additional information Mn2+, Co2+, Ni2+, Zn2+, Ca2+, Cu2+ and Co3+ can not substitute for Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH Escherichia coli enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Escherichia coli
-
 recombinant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Escherichia coli 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r
1.1.1.86 NADPH + 2-acetolactate
-
 Escherichia coli NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADPH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.86 additional information
-
 additional information
-
 Escherichia coli