Literature summary extracted from

Shematek, E.M.; Arfin, S.M.; Diven, W.F.
A kinetic study of alpha-acetohydroxy acid isomeroreductase from Salmonella typhimurium (1973), Arch. Biochem. Biophys., 158, 132-138.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.1.1.86	2-Hydroxyisovalerate	noncompetitive	Salmonella enterica subsp. enterica serovar Typhimurium
1.1.1.86	NADP+	linear non-competitive with respect to 2-acetolactate, linear competitive inhibitor of NADPH	Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.1.1.86	Mg2+	required	Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.86	2-acetolactate + NADPH	Salmonella enterica subsp. enterica serovar Typhimurium	enzyme of branched chain amino acid synthesis	2,3-dihydroxy-3-methylbutanoate + NADP+	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.86	Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.86	2-acetolactate + NADPH	enzyme of branched chain amino acid synthesis	Salmonella enterica subsp. enterica serovar Typhimurium	2,3-dihydroxy-3-methylbutanoate + NADP+	-	r
1.1.1.86	NADPH + 2-acetolactate	ordered reaction with NADPH and the acetohydroxy acid adding in that order, and dihydroxy acid released before NADP+. 4 mol of NADPH bind per mol of enzyme	Salmonella enterica subsp. enterica serovar Typhimurium	NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate	-	?

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.86	NADPH	4 mol of NADPH bind per mol of enzyme	Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.1.1.86	additional information	-	additional information	-	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)