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Literature summary extracted from
- The reductoisomerase of Neurospora crassa (1966), J. Biol. Chem., 241, 2047-2051.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.86 | unstable in absence of NADPH and Mg2+, very unstable in Tris buffer, 2-mercaptoethanol stabilizes | Neurospora crassa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.86 | NADH | - |
Neurospora crassa |  |
| 1.1.1.86 | NADP+ | - |
Neurospora crassa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.86 | 0.16 | - |
2-aceto-2-hydroxybutyrate | pH 7.5, 25°C | Neurospora crassa | |
| 1.1.1.86 | 0.32 | - |
2-acetolactate | pH 7.5, 25°C | Neurospora crassa | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.86 | Mg2+ | required | Neurospora crassa | |
| 1.1.1.86 | Mg2+ | optimum concentration 5 mM | Neurospora crassa | |
| 1.1.1.86 | additional information | metal ions other than Mg2+ are inhibitory | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.86 | 2-acetolactate + NADPH | Neurospora crassa | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
| 1.1.1.86 | 2-acetolactate + NADPH | Neurospora crassa | enzyme of valine synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
| 1.1.1.86 | NADPH + alpha-aceto-alpha-hydroxybutyrate | Neurospora crassa | enzyme of isoleucine biosynthesis | NADP+ + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.86 | Neurospora crassa | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.1.1.86 | lipoprotein | purified enzyme contains 39-46% lipid | Neurospora crassa |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.86 | - |
Neurospora crassa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.86 | 18.5 | - |
- |
Neurospora crassa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.86 | -10°, in presence of NADPH, 5 d, 70-80% loss of activity | Neurospora crassa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.86 | 2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Neurospora crassa | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
| 1.1.1.86 | 2-acetolactate + NADPH | enzyme of valine synthesis | Neurospora crassa | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
| 1.1.1.86 | NADPH + 2-aceto-2-hydroxybutyrate | activity is 6-10times greater than that for 2-acetolactate | Neurospora crassa | NADP+ + ? | - |
? | |
| 1.1.1.86 | NADPH + 2-acetolactate | - |
Neurospora crassa | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? | |
| 1.1.1.86 | NADPH + alpha-aceto-alpha-hydroxybutyrate | enzyme of isoleucine biosynthesis | Neurospora crassa | NADP+ + ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.86 | 40 | - |
5 min, about 90% loss of activity at pH 6.2, about 55% loss of activity at pH 6.5, 35% loss of activity oH 7.0, 30% loss of activity at pH 7.5, about 85% loss of activity at pH 8.0, about 90% loss of activity at pH 8.5 | Neurospora crassa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.86 | 7.5 | - |
2-acetolactate as substrate | Neurospora crassa |
| 1.1.1.86 | 7.5 | - |
alpha-aceto-alpha-hydroxybutyrate | Neurospora crassa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.86 | 6.6 | 8.8 | pH 6.6: about 65% of maximal activity, pH 8.8: about 90% of maximal activity, reaction with 2-acetolactate, Tris buffer | Neurospora crassa |
| 1.1.1.86 | 7 | 8.5 | pH 7.0: about 60% of maximal activity, pH 8.5: about 65% of maximal activity | Neurospora crassa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.86 | 6.2 | - |
40°C, 5 min, 90% loss of activity | Neurospora crassa |
| 1.1.1.86 | 6.5 | - |
40°C, 5 min, 55% loss of activity | Neurospora crassa |
| 1.1.1.86 | 7 | - |
40°C, 5 min, 35% loss of activity | Neurospora crassa |
| 1.1.1.86 | 7.5 | - |
40°C, 5 min, 30% loss of activity | Neurospora crassa |
| 1.1.1.86 | 8 | - |
40°C, 5 min, 85% loss of activity | Neurospora crassa |
| 1.1.1.86 | 8.5 | - |
40°C, 5 min, 90% loss of activity | Neurospora crassa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.86 | NADPH | - |
Neurospora crassa | |
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Release 2023.1 (January 2023)
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