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Literature summary extracted from

  • Matsunami, H.; Kawaguchi, H.; Inagaki, K.; Eguchi, T.; Kakinuma, K.; Tanaka, H.
    Overproduction and substrate specificity of 3-isopropylmalate dehydrogenase from Thiobacillus ferrooxidans (1998), Biosci. Biotechnol. Biochem., 62, 372-373.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.013
-
 3-ethylmalate
-
 Acidithiobacillus ferrooxidans
1.1.1.85 0.015
-
 (2R,3S)-3-isopropylmalate
-
 Acidithiobacillus ferrooxidans
1.1.1.85 0.05
-
 3-methylmalate
-
 Acidithiobacillus ferrooxidans
1.1.1.85 0.074
-
 3-tert-butylmalate
-
 Acidithiobacillus ferrooxidans
1.1.1.85 0.11
-
 3-isoamylmalate
-
 Acidithiobacillus ferrooxidans
1.1.1.85 7.7
-
 malate
-
 Acidithiobacillus ferrooxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Acidithiobacillus ferrooxidans key enzyme in leucine biosynthesis 2-oxoisocaproate + NADH + H+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Acidithiobacillus ferrooxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.85 overexpression in Escherichia coli Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Acidithiobacillus ferrooxidans 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ key enzyme in leucine biosynthesis Acidithiobacillus ferrooxidans 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Acidithiobacillus ferrooxidans (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 r
1.1.1.85 3-ethylmalate + NAD+
-
 Acidithiobacillus ferrooxidans 3-ethylpyruvate + NADH + CO2
-
 ?
1.1.1.85 3-isoamylmalate + NAD+
-
 Acidithiobacillus ferrooxidans 3-isoamylpyruvate + NADH + CO2
-
 ?
1.1.1.85 3-methylmalate + NAD+
-
 Acidithiobacillus ferrooxidans 3-methylpyruvate + NADH + CO2
-
 ?
1.1.1.85 3-tert-butylmalate + NAD+
-
 Acidithiobacillus ferrooxidans 3-tert-butylpyruvate + NADH + CO2
-
 ?
1.1.1.85 malate + NAD+
-
 Acidithiobacillus ferrooxidans pyruvate + NADH + H+ + CO2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.85 2.7
-
 3-tert-butylmalate
-
 Acidithiobacillus ferrooxidans
1.1.1.85 5
-
 malate
-
 Acidithiobacillus ferrooxidans
1.1.1.85 20
-
 3-isoamylmalate
-
 Acidithiobacillus ferrooxidans
1.1.1.85 28
-
 (2R,3S)-3-isopropylmalate
-
 Acidithiobacillus ferrooxidans
1.1.1.85 28
-
 3-methylmalate
-
 Acidithiobacillus ferrooxidans
1.1.1.85 38
-
 3-ethylmalate
-
 Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Acidithiobacillus ferrooxidans