Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, R.; Greer, A.; Dean, A.M.
    Redesigning secondary structure to invert coenzyme specificity in isopropylmalate dehydrogenase (1996), Proc. Natl. Acad. Sci. USA, 93, 12171-12176.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.85 S226R Km-value for NADP+ is about 40% compared to wild-type enzyme, Km-value for NAD+ is 2.6fold higher compared to wild-type enzyme Thermus thermophilus
1.1.1.85 S226R/D278K/I279Y Km-value for NADP+ is about 0.8% compared to wild-type enzyme, Km-value for NAD+ is 153fold higher compared to wild-type enzyme Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.012
-
 NAD+ pH 7.3, 21°C, wild-type enzyme Thermus thermophilus
1.1.1.85 0.014
-
 NADP+ pH 7.3, 21°C, mutant enzyme S226R/D278K/I279Y Thermus thermophilus
1.1.1.85 0.02
-
 NADP+ pH 7.3, 21°C, mutant enzyme D278K, I279T, P325Y and 395-Arg Thermus thermophilus
1.1.1.85 0.031
-
 NAD+ pH 7.3, 21°C, mutant enzyme S226R Thermus thermophilus
1.1.1.85 0.722
-
 NADP+ pH 7.3, 21°C, mutant enzyme S226R Thermus thermophilus
1.1.1.85 1.75
-
 NADP+ wild-type enzyme Thermus thermophilus
1.1.1.85 1.836
-
 NAD+ pH 7.3, 21°C, mutant enzyme S226R/D278K/I279Y Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Thermus thermophilus 2-oxoisocaproate + NADH + H+ + CO2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.85 0.09
-
 isopropylmalate pH 7.3, 21°C, reaction with NADP+, mutant enzyme S226R/D278K/I279Y Thermus thermophilus
1.1.1.85 0.09
-
 NADP+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R/D278K/I279Y Thermus thermophilus
1.1.1.85 0.15
-
 isopropylmalate pH 7.3, 21°C, reaction with NAD+, wild-type enzyme Thermus thermophilus
1.1.1.85 0.15
-
 NAD+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
1.1.1.85 0.26
-
 isopropylmalate pH 7.3, 21°C, reaction with NADP+, wild-type enzyme Thermus thermophilus
1.1.1.85 0.26
-
 NADP+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
1.1.1.85 0.48
-
 isopropylmalate pH 7.3, 21°C, reaction with NAD+, mutant enzyme S226R Thermus thermophilus
1.1.1.85 0.48
-
 NAD+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R Thermus thermophilus
1.1.1.85 0.88
-
 NADP+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R Thermus thermophilus
1.1.1.85 0.88
-
 isopropylmalate pH 7.3, 21°C, reaction with NADP+, mutant enzyme S226R Thermus thermophilus
1.1.1.85 1.91
-
 NAD+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R/D278K/I279Y Thermus thermophilus
1.1.1.85 1.91
-
 isopropylmalate pH 7.3, 21°C, reaction with NAD+, mutant enzyme S226R/D278K/I279Y Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Thermus thermophilus