EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.85 | expression in Escherichia coli using pBR322 as a vector | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | Thermus thermophilus | enzyme of leucine biosynthesis | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.85 | Thermus thermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.85 | partial | Thermus thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.85 | additional information | - |
- |
Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | enzyme of leucine biosynthesis | Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.85 | 70 | 80 | - |
Thermus thermophilus |
1.1.1.85 | 80 | - |
- |
Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.85 | NAD+ | - |
Thermus thermophilus |