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Literature summary extracted from

  • Sekiguchi, T.; Harada, Y.; Shishido, K.; Nosoh, Y.
    Cloning of beta-isopropylmalate dehydrogenase gene from Bacillus coagulans in Escherichia coli and purification and properties of the enzyme (1984), Biochim. Biophys. Acta, 788, 267-273.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.85 expression in Escherichia coli C6000 using pBR322 as a vector plasmid Weizmannia coagulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.85 Ca2+ 0.5 mM, 32% inhibition Weizmannia coagulans
1.1.1.85 Cu2+ 0.5 mM, 77% inhibition Weizmannia coagulans
1.1.1.85 Fe2+ 0.5 mM, 41% inhibition Weizmannia coagulans
1.1.1.85 K+ 250 mM KCl, 23% inhibition Weizmannia coagulans
1.1.1.85 NaCl 250 mM, 48% inhibition Weizmannia coagulans
1.1.1.85 PCMB 0.2 mM, 90% inhibition Weizmannia coagulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.85 Cd2+ 0.5 mM, slight activation Weizmannia coagulans
1.1.1.85 Co2+ 0.5 mM, activates Weizmannia coagulans
1.1.1.85 Mg2+ 0.5 mM, activates Weizmannia coagulans
1.1.1.85 Mn2+ 0.5 mM, activates Weizmannia coagulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.85 44000
-
 2 * 44000, monomer-dimer equilibrium in aqueous solution, SDS-PAGE Weizmannia coagulans
1.1.1.85 68000
-
 monomer-dimer equilibrium in aqueous solution, gel filtration Weizmannia coagulans
1.1.1.85 90000
-
 gel electrophoresis Weizmannia coagulans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Weizmannia coagulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.85 recombinant enzyme expressed in Escherichia coli Weizmannia coagulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.85 10.5
-
-
 Weizmannia coagulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Weizmannia coagulans 2-oxoisocaproate + NADH + H+ + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.85 dimer 2 * 44000, monomer-dimer equilibrium in aqueous solution, SDS-PAGE Weizmannia coagulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.85 additional information
-
 thermostabilization by LiCl, NaCl, KCl and to a lesser degree with RbCl, NH4Cl and CsCl Weizmannia coagulans
1.1.1.85 60
-
 5 min, 92% loss of activity in absence of NaCl, 6% loss of activity in presence of 1.5 M NaCl Weizmannia coagulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.85 9
-
-
 Weizmannia coagulans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Weizmannia coagulans