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Literature summary extracted from

  • Urano, N.; Kanayama, H.; Karube, I.
    Restriction mapping and partial characterization of beta-isopropylmalate dehydrogenase gene cloned from Citrobacter freundii (1985), J. Biotechnol., 2, 39-45.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.85 analysis the cloned 3-isopropylmalate dehydrogenase gene can be used as a genetic marker in constructing vectors in Citrobacter freundii Citrobacter freundii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.85 expression in Escherichia coli and Bacillus subtilis using pLS353 as a vector and PstI as an enzyme, enzyme activities in both Escherichia coli and Bacillus subtilis transformants are 4-8fold higher than those in the present strains Citrobacter freundii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Citrobacter freundii
-
 TIT 01010
-
1.1.1.85 Citrobacter freundii TIT 01010
-
 TIT 01010
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Citrobacter freundii 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Citrobacter freundii TIT 01010 2-oxoisocaproate + NADH + H+ + CO2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Citrobacter freundii