EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.47 | E96K mutant enzyme | Priestia megaterium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.47 | E96A | mutation increases thermostability at pH 6.5 | Priestia megaterium |
1.1.1.47 | E96G | mutation increases thermostability at pH 6.5 | Priestia megaterium |
1.1.1.47 | E96K | mutation increases thermostability by about 15°C at pH 6.5 | Priestia megaterium |
1.1.1.47 | Q252L | mutation increases thermostability at pH 6.5 | Priestia megaterium |
1.1.1.47 | Y253C | mutation increases thermostability at pH 6.5 | Priestia megaterium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 0.0051 | - |
NADP+ | E96K mutant | Priestia megaterium | |
1.1.1.47 | 0.0076 | - |
NADP+ | E96G mutant | Priestia megaterium | |
1.1.1.47 | 0.0093 | - |
NADP+ | E96A mutant | Priestia megaterium | |
1.1.1.47 | 0.018 | - |
NAD+ | E96K mutant | Priestia megaterium | |
1.1.1.47 | 0.026 | - |
NADP+ | Q252L mutant | Priestia megaterium | |
1.1.1.47 | 0.027 | - |
NADP+ | wild type | Priestia megaterium | |
1.1.1.47 | 0.037 | - |
NADP+ | Y253C mutant | Priestia megaterium | |
1.1.1.47 | 0.17 | - |
NAD+ | E96G mutant | Priestia megaterium | |
1.1.1.47 | 0.19 | - |
NAD+ | Q252L mutant | Priestia megaterium | |
1.1.1.47 | 0.25 | - |
NAD+ | Y253C mutant | Priestia megaterium | |
1.1.1.47 | 0.31 | - |
NAD+ | E96A mutant | Priestia megaterium | |
1.1.1.47 | 0.37 | - |
NAD+ | wild type | Priestia megaterium | |
1.1.1.47 | 2.7 | - |
beta-D-glucose | E96K mutant, coenzyme NAD+ | Priestia megaterium | |
1.1.1.47 | 4 | - |
beta-D-glucose | E96K mutant, coenzyme NADP+ | Priestia megaterium | |
1.1.1.47 | 8.7 | - |
beta-D-glucose | E96A mutant, coenzyme NAD+ | Priestia megaterium | |
1.1.1.47 | 9 | - |
beta-D-glucose | Q252L mutant, coenzyme NAD+ | Priestia megaterium | |
1.1.1.47 | 9.5 | - |
beta-D-glucose | wild type, coenzyme NAD+ | Priestia megaterium | |
1.1.1.47 | 10 | - |
beta-D-glucose | Q252L mutant, coenzyme NADP+ | Priestia megaterium | |
1.1.1.47 | 11 | - |
beta-D-glucose | wild type, coenzyme NADP+ | Priestia megaterium | |
1.1.1.47 | 13 | - |
beta-D-glucose | E96A mutant, coenzyme NADP+ | Priestia megaterium | |
1.1.1.47 | 16 | - |
beta-D-glucose | E96G mutant, coenzyme NAD+ | Priestia megaterium | |
1.1.1.47 | 18 | - |
beta-D-glucose | E96G mutant, coenzyme NADP+ | Priestia megaterium | |
1.1.1.47 | 55 | - |
beta-D-glucose | Y253C mutant, coenzyme NAD+ | Priestia megaterium | |
1.1.1.47 | 85 | - |
beta-D-glucose | Y253C mutant, coenzyme NADP+ | Priestia megaterium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.47 | Priestia megaterium | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.47 | beta-D-glucose + NAD+ | - |
Priestia megaterium | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
1.1.1.47 | beta-D-glucose + NADP+ | - |
Priestia megaterium | D-glucono-1,5-lactone + NADPH + H+ | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 40 | - |
pH 6.5, 20 min, wild-type enzyme is stable | Priestia megaterium |
1.1.1.47 | 50 | - |
pH 6.5, 20 min, complete inactivation of wild-type enzyme, mutant enzyme E96K, E96G, E96A, Q252L and Y253C are stable | Priestia megaterium |
1.1.1.47 | 55 | - |
pH 6.5, 20 min, about 65% loss of acticity of mutant enzyme Y253C, about 20% loss of activity of mutant enzyme Y252L, mutant enzyme E96K, E96G and E96A are stable | Priestia megaterium |
1.1.1.47 | 60 | - |
pH 6.5, 20 min, complete loss of activity of enzyme Y253C about 95% loss of activity of mutant enzyme Y252L, about 30% loss of activity of mutant enzyme E96G, mutant enzymes E96K and E95A are stable | Priestia megaterium |
1.1.1.47 | 65 | - |
pH 6.5, 20 min, complete loss of activity of mutant enzyme Q252L, about 50% loss of activity of mutant enzyme E96K, about 10% loss of activity of mutant enzyme E96A | Priestia megaterium |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 18 | - |
beta-D-glucose | E96K mutant, coenzyme NADP+ | Priestia megaterium | |
1.1.1.47 | 18 | - |
NADP+ | E96K mutant | Priestia megaterium | |
1.1.1.47 | 23 | - |
NAD+ | E96K mutant | Priestia megaterium | |
1.1.1.47 | 23 | - |
beta-D-glucose | E96K mutant, coenzyme NAD+ | Priestia megaterium | |
1.1.1.47 | 92 | - |
beta-D-glucose | E96G mutant, coenzyme NADP+ | Priestia megaterium | |
1.1.1.47 | 92 | - |
NADP+ | E96G mutant | Priestia megaterium | |
1.1.1.47 | 97 | - |
NAD+ | E96A mutant | Priestia megaterium | |
1.1.1.47 | 97 | - |
beta-D-glucose | E96A mutant, coenzyme NAD+ | Priestia megaterium | |
1.1.1.47 | 98 | - |
beta-D-glucose | E96A mutant, coenzyme NADP+ | Priestia megaterium | |
1.1.1.47 | 98 | - |
NADP+ | E96A mutant | Priestia megaterium | |
1.1.1.47 | 114 | - |
NAD+ | E96G mutant | Priestia megaterium | |
1.1.1.47 | 114 | - |
beta-D-glucose | E96G mutant, coenzyme NAD+ | Priestia megaterium | |
1.1.1.47 | 186 | - |
beta-D-glucose | Y253C mutant, coenzyme NADP+ | Priestia megaterium | |
1.1.1.47 | 186 | - |
NADP+ | Y253C mutant | Priestia megaterium | |
1.1.1.47 | 200 | - |
NAD+ | Y253C mutant | Priestia megaterium | |
1.1.1.47 | 200 | - |
beta-D-glucose | Y253C mutant, coenzyme NAD+ | Priestia megaterium | |
1.1.1.47 | 260 | - |
NADP+ | wild type | Priestia megaterium | |
1.1.1.47 | 260 | - |
beta-D-glucose | wild type, coenzyme NADP+ | Priestia megaterium | |
1.1.1.47 | 300 | - |
beta-D-glucose | Q252L mutant, coenzyme NADP+ | Priestia megaterium | |
1.1.1.47 | 300 | - |
NADP+ | Q252L mutant | Priestia megaterium | |
1.1.1.47 | 390 | - |
NAD+ | wild type, coenzyme NAD+ | Priestia megaterium | |
1.1.1.47 | 390 | - |
beta-D-glucose | wild type, coenzyme NAD+ | Priestia megaterium |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 4 | 8 | 30°C, 20 min, mutant enzyme Q252L is stable | Priestia megaterium |
1.1.1.47 | 4 | 10 | 30°C, 20 min, mutant enzymes E96K and E95A, less than 20% loss of activity | Priestia megaterium |
1.1.1.47 | 5 | - |
30°C, 20 min, about 55% loss of activity of mutant enzyme Y253C, about 35% loss of activity of mutant enzyme E96G and wild-type enzyme | Priestia megaterium |
1.1.1.47 | 6 | 7 | 30°C, 20 min, wild-type and mutant enzyme Y253C are stable | Priestia megaterium |
1.1.1.47 | 8 | - |
30°C, 20 min, about 50% loss of activity of mutant enzyme Y253C, about 80% loss of wild-type enzyme, about 30% loss of activity of mutant enzyme E96G and E96, about 15% loss of activity of mutant enzyme E96K | Priestia megaterium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.47 | NAD+ | - |
Priestia megaterium | |
1.1.1.47 | NADP+ | - |
Priestia megaterium |