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Literature summary extracted from

  • Nagao, T.; Mitamura, T.; Wang, X.H.; Negoro, S.; Yomo, T.; Urabe, I.; Okada, H.
    Cloning, nucleotide sequences, and enzymatic properties of glucose dehydrogenase isoenzymes from Bacillus megaterium IAM1030 (1992), J. Bacteriol., 174, 5013-5020.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.47 expressed in Escherichia coli, isoenzymes III and IV Priestia megaterium

General Stability

EC Number General Stability Organism
1.1.1.47 glucose dehydrogenase isoenzymes are stabilized in presence of 2 M NaCl. The effect is especially large for GlcDH-III, which is the most unstable enzyme Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.018
-
NADP+ pH 8.0, 30°C, isoenzyme GlcDH II Priestia megaterium
1.1.1.47 0.02
-
NADP+ pH 8.0, 30°C, isoenzyme GlcDH I Priestia megaterium
1.1.1.47 0.081
-
NAD+ pH 8.0, 30°C, isoenzyme GlcDH I Priestia megaterium
1.1.1.47 0.088
-
NAD+ pH 8.0, 30°C, isoenzyme GlcDH IV Priestia megaterium
1.1.1.47 0.16
-
NAD+ pH 8.0, 30°C, isoenzyme GlcDH III Priestia megaterium
1.1.1.47 0.99
-
NAD+ pH 8.0, 30°C, isoenzyme GlcDH II Priestia megaterium
1.1.1.47 1.9
-
NADP+ pH 8.0, 30°C, isoenzyme GlcDH IV Priestia megaterium
1.1.1.47 4.5
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH IV, coenzyme NADP+ Priestia megaterium
1.1.1.47 4.6
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH I, coenzyme NAD+ Priestia megaterium
1.1.1.47 7.9
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH I, coenzyme NADP+ Priestia megaterium
1.1.1.47 9.2
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH II, coenzyme NAD+ Priestia megaterium
1.1.1.47 9.8
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH IV, coenzyme NAD+ Priestia megaterium
1.1.1.47 12.4
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH II, coenzyme NADP+ Priestia megaterium
1.1.1.47 18.9
-
NADP+ pH 8.0, 30°C, isoenzyme GlcDH III Priestia megaterium
1.1.1.47 44
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH III, coenzyme NAD+ Priestia megaterium
1.1.1.47 135
-
beta-D-glucose pH 8.0, 30°C, isoenzyme GlcDH III, coenzyme NADP+ Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 29000
-
x * 29000, Glc-DH-I and GlcDH-IWG3, SDS-PAGE Priestia megaterium
1.1.1.47 34000
-
x * 34000, GlcDH-II and GlcDH-IV, SDS-PAGE Priestia megaterium
1.1.1.47 36000
-
x * 36000, enzyme form GlcDH-II, SDS-PAGE Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Priestia megaterium
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.47 4°C, 50 mM sodium phosphate pH 6.5, 2 M NaCl, stable Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 2-deoxy-D-glucose + NAD+
-
Priestia megaterium 2-deoxy-D-glucono-1,5-lactone + NADH + H+
-
?
1.1.1.47 2-deoxy-D-glucose + NADP+
-
Priestia megaterium 2-deoxy-D-glucono-1,5-lactone + NADPH + H+
-
?
1.1.1.47 beta-D-glucose + NAD+ although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ Priestia megaterium D-glucono-1,5-lactone + NADH + H+
-
?
1.1.1.47 beta-D-glucose + NADP+ although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ Priestia megaterium D-glucono-1,5-lactone + NADPH + H+
-
?
1.1.1.47 D-galactose + NADP+
-
Priestia megaterium D-galactono-1,5-lactone + NADPH
-
?
1.1.1.47 D-glucosamine + NAD+
-
Priestia megaterium D-glucosamino-1,5-lactone + NADH
-
?
1.1.1.47 D-glucosamine + NADP+
-
Priestia megaterium D-glucosamino-1,5-lactone + NADPH
-
?
1.1.1.47 D-mannose + NAD+
-
Priestia megaterium D-mannono-1,5-lactone + NADH
-
?
1.1.1.47 D-mannose + NADP+
-
Priestia megaterium D-mannono-1,5-lactone + NADPH
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 ? x * 29000, Glc-DH-I and GlcDH-IWG3, SDS-PAGE Priestia megaterium
1.1.1.47 ? x * 34000, GlcDH-II and GlcDH-IV, SDS-PAGE Priestia megaterium
1.1.1.47 ? x * 36000, enzyme form GlcDH-II, SDS-PAGE Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.47 30
-
20 min, isoenzyme GlcDH-III, without NaCl, about 55% loss of activity Priestia megaterium
1.1.1.47 45
-
20 min, isoenzyme GlcDH-IV, without NaCl, about 35% loss of activity, isoenzyme IWG3, without NaCl, about 10% loss of activity Priestia megaterium
1.1.1.47 50
-
20 min, isoenzyme GlcDH-IWG3, without NaCl, complete loss of activity, isoenzymes GlcDH-II and GlcDH-IWG3 are stable in presence of 2 M NaCl Priestia megaterium
1.1.1.47 65
-
20 min, in presence of 2 M NaCl, isoenzyme GlcDH-III loses about 30% of initial activity, isoenzyme GlcDH-IV loses about 10% of initial activity, isoenzyme IWG2 is stable Priestia megaterium
1.1.1.47 70
-
20 min, presence of 2 mM NaCl, complete inactivation of isoenzyme GlcDH-III and GlcDH-IV, isoenzyme GlcDH-IWG3 loses 30% of initial activity Priestia megaterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.47 120
-
beta-D-glucose pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH I Priestia megaterium
1.1.1.47 140
-
beta-D-glucose pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH II Priestia megaterium
1.1.1.47 190
-
beta-D-glucose pH 8.0, 30°C, coenzyme NAD+, isoenzymes GlcDH I and GlcDH II Priestia megaterium
1.1.1.47 420
-
beta-D-glucose pH 8.0, 30°C, coenzyme NAD+, isoenzyme GlcDH IV Priestia megaterium
1.1.1.47 430
-
beta-D-glucose pH 8.0, 30°C, coenzyme NAD+, isoenzyme GlcDH III Priestia megaterium
1.1.1.47 462
-
beta-D-glucose pH 8.0, 30°C, coenzyme NADP, isoenzyme GlcDH IV Priestia megaterium
1.1.1.47 802
-
beta-D-glucose pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH III Priestia megaterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.47 additional information
-
isoenzyme GlcDHIII has no stable pH-region without NaCl Priestia megaterium
1.1.1.47 5 10 20 min, in presence of 2 M NaCl, isoenzyme GlcDH-III is stable Priestia megaterium
1.1.1.47 5.5 10 20 min, in presence of 2 M NaCl, isoenzyme GlcDH-V is stable Priestia megaterium
1.1.1.47 6 9 20 min, in presence of 2 M NaCl, isoenzyme GlcDH-IWG3 is stable Priestia megaterium
1.1.1.47 6.5 7 20 min, without NaCl, isoenzyme GlcDH-IWG3 and GlcDH-III are stable Priestia megaterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NAD+ although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ Priestia megaterium
1.1.1.47 NADP+ although they can utilize both NAD+ and NADP+: GlcDH-III and GlcDH-IV prefer NAD+, and GlcDH-I and GlcDH-II prefer NADP+ Priestia megaterium