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Literature summary extracted from

  • Tranulis, M.A.; Christophersen, B.; Borrebaek, B.
    Glucose dehydrogenase in beef (Bos taurus) and rainbow trout (Oncorhynchus mykiss) liver: a comparative study (1994), Comp. Biochem. Physiol. B, 109, 427-435.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 30
-
 NAD+
-
 Oncorhynchus mykiss
1.1.1.47 48
-
 NAD+
-
 Bos taurus
1.1.1.47 63
-
 NADP+
-
 Bos taurus
1.1.1.47 105
-
 NADP+
-
 Oncorhynchus mykiss

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.47 endoplasmic reticulum enzyme resides on the luminal side of liver microsomes Bos taurus 5783
-
1.1.1.47 endoplasmic reticulum enzyme resides on the luminal side of liver microsomes Oncorhynchus mykiss 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 90000
-
 2 * 90000, SDS-PAGE Oncorhynchus mykiss
1.1.1.47 230000
-
 gel filtration Oncorhynchus mykiss
1.1.1.47 230000
-
 native PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Bos taurus
-
-
-
1.1.1.47 Oncorhynchus mykiss
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 DEAE-Sepharose, 2',5'-ADP-Sepharose, Sephacryl Bos taurus
1.1.1.47 DEAE-Sepharose, 2',5'-ADP-Sepharose, Sephacryl Oncorhynchus mykiss

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.47 liver
-
 Bos taurus
-
1.1.1.47 liver
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.47 6.2
-
-
 Oncorhynchus mykiss
1.1.1.47 33.6
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 beta-D-glucose + NAD+
-
 Bos taurus D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 beta-D-glucose + NAD+
-
 Oncorhynchus mykiss D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 beta-D-glucose + NADP+
-
 Bos taurus D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 beta-D-glucose + NADP+
-
 Oncorhynchus mykiss D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 D-glucose + NAD+
-
 Bos taurus D-glucono-1,5-lactone + NADH + H+
-
 r
1.1.1.47 D-glucose + NAD+
-
 Oncorhynchus mykiss D-glucono-1,5-lactone + NADH + H+
-
 r
1.1.1.47 D-glucose + NADP+
-
 Bos taurus D-glucono-1,5-lactone + NADPH + H+
-
 r
1.1.1.47 D-glucose + NADP+
-
 Oncorhynchus mykiss D-glucono-1,5-lactone + NADPH + H+
-
 r
1.1.1.47 D-glucose 6-phosphate + NADP+
-
 Bos taurus D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?
1.1.1.47 D-glucose 6-phosphate + NADP+
-
 Oncorhynchus mykiss D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 dimer 2 * 90000, SDS-PAGE Oncorhynchus mykiss

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.47 1.5
-
 D-glucose 6-phosphate
-
 Bos taurus
1.1.1.47 1.5
-
 D-glucose 6-phosphate coenzyme NADP+ Oncorhynchus mykiss
1.1.1.47 1.6
-
 D-glucose 6-phosphate coenzyme NAD+ Bos taurus
1.1.1.47 11.1
-
 D-glucose coenzyme NAD+ Oncorhynchus mykiss
1.1.1.47 12.7
-
 D-glucose 6-phosphate coenzyme NADP+ Oncorhynchus mykiss
1.1.1.47 14.2
-
 D-glucose 6-phosphate coenzyme NAD+ Oncorhynchus mykiss
1.1.1.47 14.7
-
 D-glucose coenzyme NADP+ Oncorhynchus mykiss
1.1.1.47 41
-
 D-glucose coenzyme NADP+ Bos taurus
1.1.1.47 45.9
-
 D-glucose coenzyme NAD+ Bos taurus