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Literature summary extracted from
- A novel NADP+ dependent serine dehydrogenase from Agrobacterium tumefaciens (1997), Biosci. Biotechnol. Biochem., 61, 152-157.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.276 | 2-methylmalonate | competitive against L-serine, noncompetitive against NADP+ | Agrobacterium tumefaciens |  |
| 1.1.1.276 | Co2+ | weak | Agrobacterium tumefaciens | |
| 1.1.1.276 | D-cysteine | competitive against L-serine, noncompetitive against NADP+ | Agrobacterium tumefaciens | |
| 1.1.1.276 | Hg2+ | strong | Agrobacterium tumefaciens | |
| 1.1.1.276 | L-cysteine | competitive against L-serine, noncompetitive against NADP+ | Agrobacterium tumefaciens | |
| 1.1.1.276 | malonate | competitive against L-serine, noncompetitive against NADP+ | Agrobacterium tumefaciens | |
| 1.1.1.276 | additional information | no inhibition by EDTA, sodium arsenate, o-phenethroline, alpha,alpha'-dipyridyl and very slight inhibition by sodium azide | Agrobacterium tumefaciens | |
| 1.1.1.276 | N-ethylmaleimide | - |
Agrobacterium tumefaciens | |
| 1.1.1.276 | p-chloromercuribenzoate | strong | Agrobacterium tumefaciens | |
| 1.1.1.276 | Tartronate | competitive against L-serine, noncompetitive against NADP+ | Agrobacterium tumefaciens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.276 | 0.029 | - |
NADP+ | pH 9.0, 30°C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 42 | - |
L-serine | pH 9.0, 30 °C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 44 | - |
D-serine | pH 9.0, 30 °C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 54 | - |
L-Glycerate | pH 9.0, 30 °C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 56 | - |
D-glycerate | pH 9.0, 30 °C | Agrobacterium tumefaciens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.276 | additional information | no effect with Cu2+, Mg2+, Mn2+, Zn2+, Fe2+ | Agrobacterium tumefaciens |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.276 | 100000 | - |
gel filtration | Agrobacterium tumefaciens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.276 | L-serine + NADP+ | Agrobacterium tumefaciens | - |
2-aminomalonate semialdehyde + NADPH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.276 | Agrobacterium tumefaciens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.276 | - |
Agrobacterium tumefaciens |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.1.1.276 | L-serine + NADP+ = 2-aminoacetaldehyde + CO2 + NADPH + H+ | sequential mechanism | Agrobacterium tumefaciens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.276 | 45.3 | - |
purified enzyme | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.276 | 2-methyl-DL-serine + NADP+ | 8.7% activity compared to L-serine | Agrobacterium tumefaciens | 2-amino-2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
| 1.1.1.276 | 3-hydroxypropanoate + NADP+ | 1.6% activity compared to L-serine | Agrobacterium tumefaciens | 3-oxopropanoate + NADPH | - |
? | |
| 1.1.1.276 | D-3-hydroxyisobutyrate + NADP+ | 3.5% activity compared to L-serine | Agrobacterium tumefaciens | 2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
| 1.1.1.276 | D-glycerate + NADP+ | 12.9% activity compared to L-serine | Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.1.276 | D-serine + NADP+ | 55.2% activity compared to L-serine | Agrobacterium tumefaciens | 2-aminomalonate semialdehyde + NADPH | - |
? | |
| 1.1.1.276 | L-3-hydroxyisobutyrate + NADP+ | 8.5% activity compared to L-serine | Agrobacterium tumefaciens | 2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
| 1.1.1.276 | L-glycerate + NADP+ | 11.3% activity compared to L-serine | Agrobacterium tumefaciens | ? | - |
? | |
| 1.1.1.276 | L-serine + NADP+ | - |
Agrobacterium tumefaciens | 2-aminomalonate semialdehyde + NADPH | - |
? | |
| 1.1.1.276 | L-serine + NADP+ | best substrate | Agrobacterium tumefaciens | 2-aminomalonate semialdehyde + NADPH | - |
? | |
| 1.1.1.276 | additional information | no activity with 4-hydroxybutyrate, DL-2-hydroxybutyrate, DL-2-hydroxyisobutyrate, DL-2-hydroxycaproate, DL-2-hydroxyisocaproate, 3-phosphoglycerate, DL-lactate, L-malate, glycollate, glycerol, D-threonine, DL-threo-phenylserine, DL-homoserine, DL-isoserine, L-alanine, beta-alanine, L-aspartate, L-glutamate, L-valine, L-leucine, L-methionine, L-lysine, L-phenylalanine, and glycine | Agrobacterium tumefaciens | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.276 | 30 | - |
assay at | Agrobacterium tumefaciens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.276 | 35 | - |
stable at | Agrobacterium tumefaciens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.276 | 9.1 | - |
- |
Agrobacterium tumefaciens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.276 | 7 | 8 | stable at | Agrobacterium tumefaciens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.276 | NADP+ | dependent on | Agrobacterium tumefaciens | |
| 1.1.1.276 | NADP+ | NAD+ cannot replace NADP+ | Agrobacterium tumefaciens | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.276 | 0.067 | - |
L-cysteine | versus L-serine, pH 9.0, 30°C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 0.21 | - |
L-cysteine | versus NADP+, pH 9.0, 30°C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 0.59 | - |
D-cysteine | versus L-serine, pH 9.0, 30°C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 9.9 | - |
D-cysteine | versus NADP+, pH 9.0, 30°C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 10 | - |
2-methylmalonate | versus NADP+, pH 9.0, 30°C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 11 | - |
malonate | versus L-serine, pH 9.0, 30°C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 12 | - |
2-methylmalonate | versus L-serine, pH 9.0, 30°C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 13 | - |
Tartronate | versus L-serine, pH 9.0, 30°C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 17 | - |
malonate | versus NADP+, pH 9.0, 30°C | Agrobacterium tumefaciens | |
| 1.1.1.276 | 18 | - |
Tartronate | versus NADP+, pH 9.0, 30°C | Agrobacterium tumefaciens | |
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