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Literature summary extracted from

  • Okada, G.; Teraoka, H.; Tsukada, K.
    Multiple species of mammalian S-adenosylmethionine synthetase. Partial purification and characterization (1981), Biochemistry, 20, 934-940.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.6 Dimethylsulfoxide activates alpha and beta isoenzymes from liver Rattus norvegicus
2.5.1.6 dithiothreitol required for alpha and beta isoenzymes activity, not required for gamma enzyme Rattus norvegicus
2.5.1.6 glycerol activates rat liver isoenzymes alpha and beta Rattus norvegicus
2.5.1.6 tripolyphosphates activation or inhibition, depending on isoenzyme, S-adenosylmethionine and tripolyphosphate concentration Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.6 Dimethylsulfoxide slight inhibition of gamma isoenzyme from kidney Rattus norvegicus
2.5.1.6 glycerol inhibits kidney isoenzyme gamma Rattus norvegicus
2.5.1.6 p-chloromercuribenzoate alpha and beta isoenzymes completely inhibited, gamma isoenzyme slightly inhibited Rattus norvegicus
2.5.1.6 tripolyphosphate activation or inhibition, depending on isoenzyme, S-adenosylmethionine and tripolyphosphate concentration Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.6 cytosol
-
Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.6 K+ absolute requirement, cannot be replaced by Na+ Rattus norvegicus
2.5.1.6 Mg2+ absolute requirement, cannot be replaced by Mn2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.6 160000
-
beta isoenzyme from liver, gel filtration Rattus norvegicus
2.5.1.6 190000
-
gamma isoenzyme from kidney, gel filtration Rattus norvegicus
2.5.1.6 210000
-
alpha isoenzyme, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.6 gamma isoenzyme from kidney Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.6 kidney gamma isoenzyme in kidney enzyme Rattus norvegicus
-
2.5.1.6 liver alpha and beta isozymes Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.6 3
-
beta isoenzyme from liver Rattus norvegicus
2.5.1.6 7.5
-
alpha isoenzyme from liver Rattus norvegicus
2.5.1.6 39
-
gamma isoenzyme from liver Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.5.1.6 0°C, 50 mM Tris/HCl, pH 7.8, 20% v/v glycerol, 0.2 mM DTT, 0.1 mM EDTA, 10 mM MgCl2, 0.1 M KCl, partially purified liver enzymes stable for at least 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.6 2'-deoxy-ATP + L-methionine + H2O
-
Rattus norvegicus ?
-
?
2.5.1.6 3'-deoxy-ATP + L-methionine + H2O
-
Rattus norvegicus ?
-
?
2.5.1.6 ATP + L-methionine + H2O
-
Rattus norvegicus S-adenosyl-L-methionine + phosphate + diphosphate
-
?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.6 7.5 9.5 about 70-80% of maximal activity at pH 7.5 and 9.5 Rattus norvegicus