EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.6 | Dimethylsulfoxide | activates alpha and beta isoenzymes from liver | Rattus norvegicus | |
2.5.1.6 | dithiothreitol | required for alpha and beta isoenzymes activity, not required for gamma enzyme | Rattus norvegicus | |
2.5.1.6 | glycerol | activates rat liver isoenzymes alpha and beta | Rattus norvegicus | |
2.5.1.6 | tripolyphosphates | activation or inhibition, depending on isoenzyme, S-adenosylmethionine and tripolyphosphate concentration | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.6 | Dimethylsulfoxide | slight inhibition of gamma isoenzyme from kidney | Rattus norvegicus | |
2.5.1.6 | glycerol | inhibits kidney isoenzyme gamma | Rattus norvegicus | |
2.5.1.6 | p-chloromercuribenzoate | alpha and beta isoenzymes completely inhibited, gamma isoenzyme slightly inhibited | Rattus norvegicus | |
2.5.1.6 | tripolyphosphate | activation or inhibition, depending on isoenzyme, S-adenosylmethionine and tripolyphosphate concentration | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.5.1.6 | cytosol | - |
Rattus norvegicus | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.6 | K+ | absolute requirement, cannot be replaced by Na+ | Rattus norvegicus | |
2.5.1.6 | Mg2+ | absolute requirement, cannot be replaced by Mn2+ | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.6 | 160000 | - |
beta isoenzyme from liver, gel filtration | Rattus norvegicus |
2.5.1.6 | 190000 | - |
gamma isoenzyme from kidney, gel filtration | Rattus norvegicus |
2.5.1.6 | 210000 | - |
alpha isoenzyme, gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.6 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.6 | gamma isoenzyme from kidney | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.6 | kidney | gamma isoenzyme in kidney enzyme | Rattus norvegicus | - |
2.5.1.6 | liver | alpha and beta isozymes | Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.6 | 3 | - |
beta isoenzyme from liver | Rattus norvegicus |
2.5.1.6 | 7.5 | - |
alpha isoenzyme from liver | Rattus norvegicus |
2.5.1.6 | 39 | - |
gamma isoenzyme from liver | Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.5.1.6 | 0°C, 50 mM Tris/HCl, pH 7.8, 20% v/v glycerol, 0.2 mM DTT, 0.1 mM EDTA, 10 mM MgCl2, 0.1 M KCl, partially purified liver enzymes stable for at least 1 month | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.6 | 2'-deoxy-ATP + L-methionine + H2O | - |
Rattus norvegicus | ? | - |
? | |
2.5.1.6 | 3'-deoxy-ATP + L-methionine + H2O | - |
Rattus norvegicus | ? | - |
? | |
2.5.1.6 | ATP + L-methionine + H2O | - |
Rattus norvegicus | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.6 | 7.5 | 9.5 | about 70-80% of maximal activity at pH 7.5 and 9.5 | Rattus norvegicus |