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Literature summary extracted from

  • Kotb, M.; Kredich, N.M.
    S-Adenosylmethionine synthetase from human lymphocytes. Purification and characterization (1985), J. Biol. Chem., 260, 3923-3930.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.6 ATP
-
 Homo sapiens
2.5.1.6 Dimethylsulfoxide
-
 Homo sapiens
2.5.1.6 diphosphate
-
 Homo sapiens
2.5.1.6 L-methionine
-
 Homo sapiens
2.5.1.6 additional information overview of the regulatory properties, effect of L-methionine analogues and influence of L-methionine concentration on activating and inhibiting effects, effect of tripolyphosphate and p-hydroxymercuribenzoate Homo sapiens
2.5.1.6 p-chloromercuribenzoate
-
 Homo sapiens
2.5.1.6 phosphate
-
 Homo sapiens
2.5.1.6 S-adenosylmethionine non competitive with ATP at low methionine concentration Homo sapiens
2.5.1.6 tripolyphosphate competitive with ATP and non competitive with L-methionine Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.6 0.031
-
 ATP
-
 Homo sapiens
2.5.1.6 3.3
-
 L-methionine
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.6 Mg2+ Mg2+ in excess of that bound to ATP and EDTA is required for optimal activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.6 185000
-
 equilibrium sedimentation studies Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.6 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.6 ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine steady state ordered Bi Ter mechanism with ATP adding before L-methionine and S-adenosylmethionine being the first product released, random release of phosphate and diphosphate Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.6 lymphocyte from chronic leukemia cells Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.6 additional information
-
-
 Homo sapiens
2.5.1.6 12.2
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.5.1.6 -70°C, less than 10% loss of activity after 3 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.6 2'-deoxy-ATP + L-methionine + H2O
-
 Homo sapiens ?
-
 ?
2.5.1.6 3'-deoxy-ATP + L-methionine + H2O
-
 Homo sapiens ?
-
 ?
2.5.1.6 ATP + L-methionine + H2O
-
 Homo sapiens S-adenosyl-L-methionine + phosphate + diphosphate
-
 ?
2.5.1.6 tripolyphosphate + H2O tripolyphosphatase activity Homo sapiens diphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.6 ?
-
 Homo sapiens
2.5.1.6 tetramer heterotetramer with subunit composition alpha-alpha'-beta2, alpha2beta2 or alpha'2beta2, where alpha, alpha', and beta are chains of molecular weight 53000, 51000 and 38000 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.6 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.6 22
-
 stable for several hours, if protein concentration is above 0.1 mg/ml and ionic strength is at least 50 mM Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.6 7.5 8.5
-
 Homo sapiens