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Literature summary extracted from

  • Porcelli, M.; Cacciapuoti, G.; Carteni-Farina, M.; Gambacorta, A.
    S-adenosylmethionine synthetase in the thermophilic archaebacterium Sulfolobus solfataricus. Purification and characterization of two isoforms (1988), Eur. J. Biochem., 177, 273-280.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.6 additional information no requirement for reducing agents Saccharolobus solfataricus
2.5.1.6 S-adenosylmethionine activates the tripolyphosphatase activity of A isoform Saccharolobus solfataricus

General Stability

EC Number General Stability Organism
2.5.1.6 ATP, preincubation protects against thermal inactivation Saccharolobus solfataricus
2.5.1.6 glycerol, 10% stabilizes Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.6 CTP 20 mM, 37% inhibition, S-adenosylmethionine synthetase B; 20 mM, 40% inhibition, S-adenosylmethionine synthetase A Saccharolobus solfataricus
2.5.1.6 cycloleucine 25 mM, 56% inhibition, S-adenosylmethionine synthetase A; inhibits only at sub saturating concentrations of methionine Saccharolobus solfataricus
2.5.1.6 diphosphate 20 mM, 30% inhibition, S-adenosylmethionine synthetase A; 20 mM, 49% inhibition, S-adenosylmethionine synthetase B Saccharolobus solfataricus
2.5.1.6 GTP 20 mM, 50% inhibition, S-adenosylmethionine synthetase B; 20 mM, 56% inhibition, S-adenosylmethionine synthetase A Saccharolobus solfataricus
2.5.1.6 additional information S-adenosyl(5')-3-methylthiopropylamine does not inhibit Saccharolobus solfataricus
2.5.1.6 phosphate 10 mM, 19% inhibition, S-adenosylmethionine synthetase B; 10 mM, 45% inhibition, S-adenosylmethionine synthetase A Saccharolobus solfataricus
2.5.1.6 S-adenosyl-L-homocysteine not inhibitory Saccharolobus solfataricus
2.5.1.6 S-adenosylmethionine inhibits the A form but not the B form; non-competitive, S-adenosylmethionine synthetase A; slight inhibition of S-adenosylmethionine synthetase B Saccharolobus solfataricus
2.5.1.6 Tetrapolyphosphate 10 mM, 40% inhibition, S-adenosylmethionine synthetase B; 10 mM, 50% inhibition, S-adenosylmethionine synthetase A Saccharolobus solfataricus
2.5.1.6 tripolyphosphate 1.0 mM, 57% inhibition, S-adenosylmethionine synthetase A; 1.0 mM, 62% inhibition, S-adenosylmethionine synthetase B Saccharolobus solfataricus
2.5.1.6 UTP
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.6 0.01
-
 L-methionine isoenzyme A Saccharolobus solfataricus
2.5.1.6 0.01
-
 L-methionine S-adenosylmethionine synthetase A Saccharolobus solfataricus
2.5.1.6 0.02
-
 L-methionine isoenzyme B Saccharolobus solfataricus
2.5.1.6 0.024
-
 L-methionine S-adenosylmethionine synthetase B Saccharolobus solfataricus
2.5.1.6 0.05
-
 ATP isoenzyme A Saccharolobus solfataricus
2.5.1.6 0.065
-
 ATP S-adenosylmethionine synthetase A Saccharolobus solfataricus
2.5.1.6 0.15
-
 ATP isoenzyme B Saccharolobus solfataricus
2.5.1.6 0.156
-
 ATP S-adenosylmethionine synthetase B Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.6 cytosol
-
 Saccharolobus solfataricus 5829
-
2.5.1.6 cytosol S-adenosylmethionine synthetase A Saccharolobus solfataricus 5829
-
2.5.1.6 cytosol S-adenosylmethionine synthetase B Saccharolobus solfataricus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.6 Co2+ can replace Mg2+ with lower relative activity Saccharolobus solfataricus
2.5.1.6 Co2+ can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase B Saccharolobus solfataricus
2.5.1.6 Mg2+ maximal activation at 40 mM Saccharolobus solfataricus
2.5.1.6 Mg2+ S-adenosylmethionine synthetase A is absolutely dependent upon the presence of Mg2+, suggesting that a Mg-ATP complex functions as substrate. Maximal activity is obtained at an Mg2+ concentration of 40 mM with 10 mM ATP. 30% of the maximal reaction rate is observable at equimolar concentrations of ATP and Mg2+ (10 mM) Saccharolobus solfataricus
2.5.1.6 Mg2+ S-adenosylmethionine synthetase B is absolutely dependent upon the presence of Mg2+, suggesting that a Mg-ATP complex functions as substrate. Maximal activity is obtained at an Mg2+ concentration of 40 mM with 10 mM ATP. 30% of the maximal reaction rate is observable at equimolar concentrations of ATP and Mg2+ (10 mM) Saccharolobus solfataricus
2.5.1.6 Mn2+ can replace Mg2+ with lower relative activity Saccharolobus solfataricus
2.5.1.6 Mn2+ can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase A Saccharolobus solfataricus
2.5.1.6 Mn2+ can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase B Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.6 75000
-
 isoenzyme B, gel filtration Saccharolobus solfataricus
2.5.1.6 75000
-
 S-adenosylmethionine synthetase B, gel filtration Saccharolobus solfataricus
2.5.1.6 180000
-
 isoenzyme A, gel filtration Saccharolobus solfataricus
2.5.1.6 180000
-
 S-adenosylmethionine synthetase A, gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O Saccharolobus solfataricus
-
 S-adenosyl-L-methionine + phosphate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.6 Saccharolobus solfataricus
-
-
-
2.5.1.6 Saccharolobus solfataricus
-
 2 forms: A and B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.6 partial, S-adenosylmethionine synthetase B Saccharolobus solfataricus
2.5.1.6 two isoforms: A and B Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.6 additional information
-
-
 Saccharolobus solfataricus
2.5.1.6 0.065
-
 S-adenosylmethionine synthetase B Saccharolobus solfataricus
2.5.1.6 0.138
-
 S-adenosylmethionine synthetase A Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
2.5.1.6 -20°C, partially purified isoenzymes, 10 mM Tris/HCl, pH 8, stable for 4 weeks Saccharolobus solfataricus
2.5.1.6 -20°C, pH 8, 10 mM Tris/HCl buffer, partially purified S-adenosylmethionine synthetase A is stable for four weeks Saccharolobus solfataricus
2.5.1.6 -20°C, pH 8, 10 mM Tris/HCl buffer, partially purified S-adenosylmethionine synthetase B is stable for four weeks Saccharolobus solfataricus
2.5.1.6 4°C, isoenzyme A and B are spontaneously inactivated in 1 month and 1 week, respectively, in presence of glycerol stability increases up to 90% Saccharolobus solfataricus
2.5.1.6 4°C, S-adenosylmethionine synthetase is spontaneously inactivated in 1 month, S-adenosylmethionine synthetase A Saccharolobus solfataricus
2.5.1.6 4°C, S-adenosylmethionine synthetase is spontaneously inactivated in 1 week Saccharolobus solfataricus
2.5.1.6 are stable in buffer A at Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.6 2'-deoxy-ATP + L-methionine + H2O
-
 Saccharolobus solfataricus ?
-
 ?
2.5.1.6 3'-deoxy-ATP + L-methionine + H2O
-
 Saccharolobus solfataricus ?
-
 ?
2.5.1.6 ATP + L-methionine + H2O
-
 Saccharolobus solfataricus S-adenosyl-L-methionine + phosphate + diphosphate
-
 ?
2.5.1.6 ATP + L-methionine + H2O
-
 Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
2.5.1.6 additional information S-adenosylmethionine synthetase A exhibits tripolyphosphatase activity Saccharolobus solfataricus ?
-
 ?
2.5.1.6 additional information S-adenosylmethionine synthetase B exhibits tripolyphosphatase activity Saccharolobus solfataricus ?
-
 ?
2.5.1.6 tripolyphosphate + H2O tripolyphosphatase activity Saccharolobus solfataricus diphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.6 S-adenosylmethionine synthetase A
-
 Saccharolobus solfataricus
2.5.1.6 S-adenosylmethionine synthetase B
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.6 90
-
-
 Saccharolobus solfataricus
2.5.1.6 90
-
 S-adenosylmethionine synthetase A Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.6 75 110 about 50% of activity maximum at 75°C and 110°C Saccharolobus solfataricus
2.5.1.6 75 110 50% of maximal activity at 75°C and at 110°C Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.6 additional information
-
 preincubation with ATP protects against thermal inactivation Saccharolobus solfataricus
2.5.1.6 100
-
 30 min, 90% loss of activity Saccharolobus solfataricus
2.5.1.6 100
-
 more than 80% activity is retained in the presence of 10 mM ATP at 100°C for 30 min. GTP, ADP, AMP and methionine do not exert any protective effect, S-adenosylmethionine synthetase A Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.6 8.1
-
-
 Saccharolobus solfataricus
2.5.1.6 8.1
-
 S-adenosylmethionine synthetase A Saccharolobus solfataricus
2.5.1.6 8.1
-
 S-adenosylmethionine synthetase B Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.6 7.2 9.3 about 50% of activity maximum at pH 7.2 and 9.3 Saccharolobus solfataricus
2.5.1.6 7.2 9.3 50% of maximal activity at pH 7.2 and at pH 9.3, S-adenosylmethionine synthetase A Saccharolobus solfataricus
2.5.1.6 7.2 9.3 50% of maximal activity at pH 7.2 and at pH 9.3, S-adenosylmethionine synthetase B Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.6 0.05
-
 S-adenosylmethionine inhibits the A isoform Saccharolobus solfataricus
2.5.1.6 0.05
-
 S-adenosylmethionine S-adenosylmethionine synthetase A Saccharolobus solfataricus